SLC7A5 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC7A5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 13752-1-AP

Synonyms

4F2 light chain, 4F2LC, CD98 light chain, CD98LC, D16S469E, E16, hLAT1, Integral membrane protein E16, LAT1, MPE16, SLC7A5

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Validation Data Gallery

• 

  WB analysis of mouse lung using 13752-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13752-1-AP (SLC7A5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13752-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13752-1-AP (SLC7A5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13752-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13752-1-AP (SLC7A5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse lung tissue, human placenta tissue, MCF-7 cells
• Positive IHC detected in: human lung tissue, mouse lung tissue, mouse liver tissue, human testis tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Product Information

13752-1-AP targets SLC7A5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, cow
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC7A5 fusion protein Ag4694
• Full Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
• Calculated Molecular Weight: 507 aa, 55 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC039692
• Gene Symbol: SLC7A5
• Gene ID (NCBI): 8140
• RRID: AB_2190973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Mol Life Sci; Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma.; Authors - Bo Zhang
human	IHC	J Nucl Med; PMID: 29700126; Authors - Deep Hathi
mouse	WB	Mol Metab; Resistance exercise enhances long-term mTORC1 sensitivity to leucine; Authors - Gommaar D'Hulst
human	WB	ACS Chem Neurosci; Baicalein Delays H2O2-Induced Astrocytic Senescence through Inhibition of Senescence-Associated Secretory Phenotype (SASP), Suppression of JAK2/STAT1/NF-κB Pathway, and Regulation of Leucine Metabolism.; Authors - Li Gao
human	WB,IHC	J Biol Chem; GSE1 predicts poor survival outcome in gastric cancer patients by SLC7A5 enhancement of tumor growth and metastasis.; Authors - Keshuo Ding
human	IHC	Br J Cancer; ASCT2 (SLC1A5)-dependent glutamine uptake is involved in the progression of head and neck squamous cell carcinoma.; Authors - Ze Zhang

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