Tested Applications
| Positive WB detected in | HUVEC cells, NIH/3T3 cells |
| Positive IHC detected in | human colon cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13823-1-AP targets SLC7A6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4924 Product name: Recombinant human SLC7A6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 217-515 aa of BC028216 Sequence: GHSEHFQDAFEGSSWDMGNLSLALYSALFSYSGWDTLNFVTEEIKNPERNLPLAIGISMPIVTLIYILTNVAYYTVLNISDVLSSDAVAVTFADQTFGMFSWTIPIAVALSCFGGLNASIFASSRLFFVGSREGHLPDLLSMIHIERFTPIPALLFNCTMALIYLIVEDVFQLINYFSFSYWFFVGLSVVGQLYLRWKEPKRPRPLKLSVFFPIVFCICSVFLVIVPLFTDTINSLIGIGIALSGVPFYFMGVYLPESRRPLFIRNVLAAITRGTQQLCFCVLTELDVAEEKKDERKTD Predict reactive species |
| Full Name | solute carrier family 7 (cationic amino acid transporter, y+ system), member 6 |
| Calculated Molecular Weight | 515 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC028216 |
| Gene Symbol | SLC7A6 |
| Gene ID (NCBI) | 9057 |
| RRID | AB_2877978 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92536 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC7A6 antibody 13823-1-AP | Download protocol |
| WB protocol for SLC7A6 antibody 13823-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SLC7A6 Polyclonal antibody (Cat# 13823-1-AP) has 7 citations published in Int J Biol Macromol, Haematologica, Neoplasia, Poult Sci, Front Physiol, J Anim Sci, and Reprod Toxicol. Research fields span ferroptosis and GSSG transport regulation, acute leukemia, bladder cancer and glutamine metabolism, zinc and amino acid transporter expression in broilers, and placental function/neural tube defects in mice. Applications include WB and IHC across human, chicken, and mouse species.
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Structural characterization and functional role of SLC7A6 in GSSG transport and Ferroptosis regulation.
| ||
Poult Sci The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers | ||
Front Physiol Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers | ||
J Anim Sci Zinc proteinate with moderate chelation strength enhances zinc absorption by upregulating the expression of zinc and amino acid transporters in primary cultured duodenal epithelial cells of broiler embryos |
