SLCO1B3/OATP1B3 Monoclonal antibody

SLCO1B3/OATP1B3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

1D9A4

Cat no : 66381-1-Ig

Synonyms

LST 2, LST 3TM13, LST2, LST3, OATP 8, OATP1B3, OATP8, Organic anion transporter 8, SLC21A8, SLCO1B3

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Validation Data Gallery

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  WB analysis of HepG2 using 66381-1-Ig

  Western blot analysis of SLCO1B3/OATP1B3 in various cell lines using Proteintech antibody 66381-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66381-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66381-1-Ig (SLCO1B3/OATP1B3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66381-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66381-1-Ig (SLCO1B3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66381-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLCO1B3/OATP1B3 antibody (66381-1-Ig, Clone: 1D9A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, SMMC-7721 cells, COLO 320 cells, Caco-2 cells, SW 1990 cells, BxPC-3 cells, PC-3 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

66381-1-Ig targets SLCO1B3/OATP1B3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLCO1B3/OATP1B3 fusion protein Ag19033
• Full Name: solute carrier organic anion transporter family, member 1B3
• Calculated Molecular Weight: 702 aa, 77 kDa
• Observed Molecular Weight: 68-77 kDa
• GenBank Accession Number: BC105597
• Gene Symbol: SLCO1B3
• Gene ID (NCBI): 28234
• RRID: AB_2881757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLCO1B3, also known as OATP8 or OATP1B3, is a member of Organic Anion Transporting Polypeptides (OATPs) which are sodium-independent organic anion transporters mediating the uptake of a wide range of structurally diverse endogenous and exogenous compounds including bile acids, hormone conjugates, peptides, toxins, as well as a multitude of therapeutic drugs. SLCO1B3 is specifically expressed in liver under normal condition, while its expression has also been observed in cancer tissues like colon, prostate and pancreas. It corresponds to Oatp1b2 in mice, with the gene symbol Slco1b2. The mouse genome encodes only a single transporter gene in the OATP1B subfamily, because SLCO1B1 and SLCO1B3 arose in primates by gene duplication after divergence from rodents. The molecular mass of SLCO1B3 is highly dependent on the glycosylation modification (deglycosylated form of 65 kDa, fully-glycosylated form of 120 kDa).

Publications

Species	Application	Title
human	WB	Aging (Albany NY); SLCO1B3 promotes colorectal cancer tumorigenesis and metastasis through STAT3.; Authors - Lianghui Zhi
human	WB	Nat Commun; Identification of indocyanine green as a STT3B inhibitor against mushroom α-amanitin cytotoxicity; Authors - Bei Wang
human	WB	Bull Exp Biol Med; The Effect of Original Russian Neurotropic Drugs on Organic Anion Transporting Polypeptides OATP1B1 and OATP1B3; Authors - P D Erokhina
human	WB	Aging (Albany NY); Comprehensive analysis of scRNA-seq and bulk RNA-seq reveal the characteristics of disulfidptosis and a prognostic signature in BLCA; Authors - Hao Deng
human	WB	Bull Exp Biol Med; Regulation of the Organic Anion Transporting Polypeptide 1B3 (OATP1B3) by Sex Hormones; Authors - A A Slepnev
	WB	Sci Rep; OATP1B3 (699G>A) and CYP2C9*2, *3 significantly influenced the transport and metabolism of glibenclamide and glipizide.; Authors - Fayou Yang