Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20217-1-AP targets SLIT2-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | slit homolog 2 (Drosophila) |
| Calculated Molecular Weight | 170 kDa |
| Observed Molecular Weight | 130-140 kDa, 200 kDa |
| GenBank Accession Number | NM_004787 |
| Gene Symbol | SLIT2 |
| Gene ID (NCBI) | 9353 |
| RRID | AB_10805766 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94813 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLIT2, also named as SLIL3, is thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development it is involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development, SLIT2 may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. SLIT2 may be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system it appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, it collapses and repels retinal ganglion cell growth cones. SLIT2 seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. It seems to be involved in regulating leukocyte migration. The antibody is specific to SLIT2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SLIT2-Specific antibody 20217-1-AP | Download protocol |
| IHC protocol for SLIT2-Specific antibody 20217-1-AP | Download protocol |
| WB protocol for SLIT2-Specific antibody 20217-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-SLIT2 antibody (Cat# 20217-1-AP) has 31 citations published in journals including Nature, Nat Cell Biol, Nat Commun, Neuron, J Clin Invest, Cell Death Dis, and Cell Rep. Associated research fields span cancer metastasis, axon guidance and neural development, fibrosis, angiogenesis, reproductive biology, and ischemic stroke.
| Species | Application | Title |
|---|---|---|
Nature Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis.
| ||
Nat Cell Biol Psychological stress-induced ALKBH5 deficiency promotes tumour innervation and pancreatic cancer via extracellular vesicle transfer of RNA | ||
Nat Commun An integrated single cell and spatial transcriptomic map of human white adipose tissue | ||
Neuron Dendrite self-avoidance requires cell-autonomous slit/robo signaling in cerebellar purkinje cells. | ||
J Clin Invest Axon guidance cue SLIT2 regulates the murine skeletal stem cell niche through sympathetic innervation. | ||
Adv Sci (Weinh) Three-Year Follow-Up of Neoadjuvant Tislelizumab with Chemotherapy in Locally Advanced Gastric and Gastroesophageal Junction Adenocarcinoma: Revealing Cancer-Associated Fibroblast Heterogeneity Corresponding to PD-1 Blockade Efficacy. |
