SLIT2-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

SLIT2-Specific Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 20217-1-AP

Synonyms

SLIL3, Slit 2, slit homolog 2 (Drosophila), Slit homolog 2 protein, SLIT2, SLIT2-Specific

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 20217-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20217-1-AP (SLIT2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20217-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20217-1-AP (SLIT2-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20217-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20217-1-AP (SLIT2-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 20217-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20217-1-AP (SLIT2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20217-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20217-1-AP (SLIT2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 20217-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20217-1-AP (SLIT2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 20217-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20217-1-AP (SLIT2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 20217-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 20217-1-AP (SLIT2-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 20217-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug SLIT2-Specific antibody (20217-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue
• Positive IHC detected in: human kidney tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• ELISA: See 1 publications below

Product Information

20217-1-AP targets SLIT2-Specific in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: slit homolog 2 (Drosophila)
• Calculated Molecular Weight: 170 kDa
• Observed Molecular Weight: 130-140 kDa, 200 kDa
• GenBank Accession Number: NM_004787
• Gene Symbol: SLIT2
• Gene ID (NCBI): 9353
• RRID: AB_10805766
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLIT2, also named as SLIL3, is thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development it is involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development, SLIT2 may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. SLIT2 may be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system it appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, it collapses and repels retinal ganglion cell growth cones. SLIT2 seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. It seems to be involved in regulating leukocyte migration. The antibody is specific to SLIT2.

Publications

Species	Application	Title
human,mouse	WB,IF	Nature; Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis.; Authors - Bernardo Tavora ; KD Validated
mouse	WB	Neuron; Dendrite self-avoidance requires cell-autonomous slit/robo signaling in cerebellar purkinje cells.; Authors - Daniel A Gibson
human	WB	Angiogenesis; Slit2/Robo1 signaling is involved in angiogenesis of glomerular endothelial cells exposed to a diabetic-like environment.; Authors - Junhui Liu ; KD Validated
mouse	WB	Front Cell Dev Biol; Deletion of Mea6 in Cerebellar Granule Cells Impairs Synaptic Development and Motor Performance.; Authors - Xin-Tai Wang
human	IF	J Transl Med; Identification and validation of smoking-related genes in lung adenocarcinoma using an in vitro carcinogenesis model and bioinformatics analysis.; Authors - Jin Wang
mouse	WB	Front Cell Neurosci; MEA6 Deficiency Impairs Cerebellar Development and Motor Performance by Tethering Protein Trafficking.; Authors - Xin-Tai Wang

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