SLITRK3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLITRK3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 21649-1-AP

Synonyms

SLIT and NTRK-like protein 3, KIAA0848

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Validation Data Gallery

• 

  WB analysis of mouse brain using 21649-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21649-1-AP

  IP result of anti-SLITRK3 (IP:21649-1-AP, 4ug; Detection:21649-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse intestine using 21649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse intestine using 21649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 21649-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 21649-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: rat small intestine tissue, mouse brain tissue, mouse intestine; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

21649-1-AP targets SLITRK3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLITRK3 fusion protein Ag16352
• Full Name: SLIT and NTRK-like family, member 3
• Calculated Molecular Weight: 977 aa, 109 kDa
• Observed Molecular Weight: 120-125 kDa
• GenBank Accession Number: BC114621
• Gene Symbol: SLITRK3
• Gene ID (NCBI): 22865
• RRID: AB_10859788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB, IHC	Neuron; Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.; Authors - Jun Li
rat	WB	Proc Natl Acad Sci U S A; Slitrks control excitatory and inhibitory synapse formation with LAR receptor protein tyrosine phosphatases.; Authors - Yim Yeong Shin YS ; KD Validated
human	WB,IF	Mol Biomed; Role of novel cancer gene SLITRK3 to activate NTRK3 in squamous cell lung cancer.; Authors - Aliccia Bollig-Fischer ; KD Validated
human	WB	Front Mol Neurosci; Human mutations in SLITRK3 implicated in GABAergic synapse development in mice; Authors - Stephanie Efthymiou