SLK Polyclonal antibody, PBS Only

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• SLK Polyclonal antibody, PBS Only (19743-1-PBS)

• 

  WB analysis using 19743-1-AP (same clone as 19743-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 19743-1-AP (same clone as 19743-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 19743-1-AP (same clone as 19743-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 19743-1-AP (same clone as 19743-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 19743-1-AP (same clone as 19743-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLK antibody (19743-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

SLK Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 19743-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

19743-1-PBS targets SLK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: STE20-like kinase (yeast)
• Calculated Molecular Weight: 143 kDa
• Observed Molecular Weight: 143-160 kDa, 210-220 kDa
• GenBank Accession Number: NM_014720
• Gene Symbol: SLK
• Gene ID (NCBI): 9748
• RRID: AB_10644278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H2G2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SLK, also named as KIAA0204 and STK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. SLK plays an important role in cell proliferation and cytoskeletal remodeling. In fibroblasts, SLK has been shown to respond to FAK/Src signaling and regulate focal adhesion turnover through Paxillin phosphorylation. Full-length SLK (~220 kDa) has also been shown to be essential for embryonic development. In myoblasts, the overexpression of a dominant negative SLK is sufficient to block myoblast fusion. (PMID: 28153048, PMID: 25882817, PMID: 23815977)