SLMAP Polyclonal antibody

• 

  WB analysis of mouse heart using 25220-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25220-1-AP (SLMAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 25220-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25220-1-AP (SLMAP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 25220-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25220-1-AP (SLMAP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

SLMAP Polyclonal Antibody for WB, ELISA

Cat No. 25220-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

KIAA1601, sarcolemma associated protein, Sarcolemmal associated protein, SLAP, SLMAP

---

Tested Applications

• Positive WB detected in: mouse heart tissue, A431 cells, mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

25220-1-AP targets SLMAP in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18162

  Product name: Recombinant human SLMAP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 479-828 aa of BC115701

  Sequence: VSLLKDDLQGAQSEIEAKQEIQHLRKELIEAQELARTSKQKCFELQALLEEERKAYRNQVEESTKQIQVLQAQLQRLHIDTENLREEKDSEITSTRDELLSARDEILLLHQAAAKVASERDTDIASLQEELKKVRAELERWRKAASEYEKEITSLQNSFQLRCQQCEDQQREEATRLQGELEKLRKEWNALETECHSLKRENVLLSSELQRQEKELHNSQKQSLELTSDLSILQMSRKELENQVGSLKEQHLRDSADLKTLLSKAENQAKDVQKEYEKTQTVLSELKLKFEMTEQEKQSITDELKQCKNNLKLLREKGNNKPWPWMPMLAALVAVTAIVLYVPGLARASP

• Full Name: sarcolemma associated protein
• Calculated Molecular Weight: 828 aa, 95 kDa
• Observed Molecular Weight: 35 kDa, 45 kDa, 63-66 kDa, 83-91 kDa
• GenBank Accession Number: BC115701
• Gene Symbol: SLMAP
• Gene ID (NCBI): 7871
• RRID: AB_2879968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14BN4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sarcolemmal membrane associated protein (SLMAP) is a tail-anchored membrane protein involved in the docking and membrane fusion process via protein-protein interactions. Deregulation of SLMAP expression plays an important role in type 2 diabetes, and changes in SLMAP expression are linked to hyperglycemia and diabetes. SLMAPs are components of cardiac membranes and have been identified as a novel regulator of cardiac function. There are several isoforms of SLMAPs with varying molecular size (35, 45, 63, and 83-91 kDa) as a result of alternative splicing. The short 35-kDa isoform is predominantly expressed in cardiac and slow-twitch skeletal muscle, while the 83- to 91-kDa isoform is ubiquitous. This antibody recognizes all of these isoforms in mouse heart lysate. (21785580, 22180652)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Thorac Cancer; Tumor suppressor effect of an antibody on xenotransplanted sarcomatoid mesothelioma cells; Authors - Masayoshi Hasegawa ; KD Validated