SLTM Polyclonal antibody

SLTM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17889-1-AP

Synonyms

SAFB-like transcription modulator, Modulator of estrogen-induced transcription, MET

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Validation Data Gallery

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  WB analysis of mouse brain using 17889-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17889-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17889-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17889-1-AP (SLTM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 17889-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17889-1-AP (SLTM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17889-1-AP

  Immunofluorescent analysis of HepG2 cells, using SLTM antibody 17889-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17889-1-AP targets SLTM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLTM fusion protein Ag12060
• Full Name: SAFB-like, transcription modulator
• Calculated Molecular Weight: 478aa,57 kDa; 1034aa,117 kDa
• Observed Molecular Weight: 135-140 kDa
• GenBank Accession Number: BC046119
• Gene Symbol: SLTM
• Gene ID (NCBI): 79811
• RRID: AB_2192729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLTM, also named as Modulator of estrogen-induced transcription, is a 1034 amino acid protein, which localizes in the nucleus. When it is overexpressed, SLTM acts as a general inhibitor of transcription that eventually leads to apoptosis. The calculated molecular weight of SLTM is 117 kDa, but modified SLTM is about 135-140 kDa.