SLU7 Polyclonal antibody

SLU7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12050-1-AP

Synonyms

9G8, hSlu7, Pre mRNA splicing factor SLU7, SLU7

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Validation Data Gallery

• 

  WB analysis of Jurkat using 12050-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12050-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 12050-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12050-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12050-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12050-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12050-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 12050-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12050-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12050-1-AP (SLU7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat pancreas using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, mouse colon tissue, K-562 cells, Y79 cells
• Positive IHC detected in: mouse pancreas tissue, human colon cancer tissue, rat pancreas tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Product Information

12050-1-AP targets SLU7 in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLU7 fusion protein Ag2680
• Full Name: SLU7 splicing factor homolog (S. cerevisiae)
• Calculated Molecular Weight: 586 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC010634
• Gene Symbol: SLU7
• Gene ID (NCBI): 10569
• RRID: AB_10663102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pre-mRNA splicing occurs in two sequential transesterification steps. SLU7 is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions.

Publications

Species	Application	Title
human	RIP	Mol Cancer; A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer; Authors - Runjie Song
human,mouse	WB,IHC,IF	Adv Sci (Weinh); G3BP1 and SLU7 Jointly Promote Immune Evasion by Downregulating MHC-I via PI3K/Akt Activation in Bladder Cancer; Authors - Xianchong Zheng ; KD Validated