SMAP2 Polyclonal antibody

SMAP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16597-1-AP

Synonyms

RP1 228H13.3, small ArfGAP2, SMAP1L, SMAP2

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Validation Data Gallery

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  WB analysis of human liver using 16597-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16597-1-AP (SMAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 16597-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16597-1-AP (SMAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 16597-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 16597-1-AP (SMAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16597-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16597-1-AP (SMAP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16597-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16597-1-AP (SMAP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16597-1-AP

  Immunofluorescent analysis of HepG2 cells, using SMAP2 antibody 16597-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human liver tissue, human colon tissue, mouse small intestine tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16597-1-AP targets SMAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMAP2 fusion protein Ag9751
• Full Name: small ArfGAP2
• Calculated Molecular Weight: 429 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC021133
• Gene Symbol: SMAP2
• Gene ID (NCBI): 64744
• RRID: AB_2066026
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.