Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13066-1-AP targets SMARCA5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3759 Product name: Recombinant human SMARCA5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 703-1052 aa of BC023144 Sequence: FTMDTESSVYNFEGEDYREKQKIAFTEWIEPPKRERKANYAVDAYFREALRVSEPKAPKAPRPPKQPNVQDFQFFPPRLFELLEKEILFYRKTIGYKVPRNPELPNAAQAQKEEQLKIDEAESLNDEELEEKEKLLTQGFTNWNKRDFNQFIKANEKWGRDDIENIAREVEGKTPEEVIEYSAVFWERCNELQDIEKIMAQIERGEARIQRRISIKKALDTKIGRYKAPFHQLRISYGTNKGKNYTEEEDRFLICMLHKLGFDKENVYDELRQCIRNSPQFRFDWFLKSRTAMELQRRCNTLITLIERENMELEEKEKAEKKKRGPKPSTQKRKMDGAPDGRGRKKKLKL Predict reactive species |
| Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 |
| Calculated Molecular Weight | 1052 aa, 122 kDa |
| Observed Molecular Weight | 122 kDa |
| GenBank Accession Number | BC023144 |
| Gene Symbol | SMARCA5 |
| Gene ID (NCBI) | 8467 |
| RRID | AB_2191854 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60264 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMARCA5, also named as SNF2H or WCRF135, is a 1052 amino acid protein, which localizes in nucleus and is overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. SMARCA5 is one of the most conserved chromatin remodeling factors. It exists in a variety of oligosubunit complexes that move DNA with respect to the histone octamer to generate regularly spaced nucleosomal arrays. Smarca5 interacts with different accessory proteins and represents a molecular motor for DNA replication, repair, and transcription.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SMARCA5 antibody 13066-1-AP | Download protocol |
| IHC protocol for SMARCA5 antibody 13066-1-AP | Download protocol |
| WB protocol for SMARCA5 antibody 13066-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SMARCA5 Polyclonal antibody (Cat# 13066-1-AP) has 9 citations published in Nucleic Acids Research, Cell Death & Differentiation, Cell Death & Disease, Journal of Experimental Medicine, Cell Communication & Signaling, Arteriosclerosis Thrombosis and Vascular Biology, Cell Reports, Journal of Cell Biology, and FASEB Journal. Research fields span DNA repair, epigenetics and melanoma, prostate cancer, hematopoietic stem cell biology, head and neck carcinoma, obesity-related macrophage inflammation, lung adenocarcinoma, paraspeckle assembly, and osteoarthritis.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res SIRT6 coordinates with CHD4 to promote chromatin relaxation and DNA repair.
| ||
Cell Death Differ GSDME acts as an epigenetic modifier to promote melanoma development via centriole biogenesis regulator PLK4. | ||
Cell Death Dis USP3 promotes DNA damage response and chemotherapy resistance through stabilizing and deubiquitinating SMARCA5 in prostate cancer | ||
J Exp Med Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis. | ||
Cell Commun Signal A positive feedback loop between BACH1 and IL-1β promotes the progression of HPV-negative head and neck squamous cell carcinoma. | ||
Arterioscler Thromb Vasc Biol Novel Long Noncoding RNA, Macrophage Inflammation-Suppressing Transcript (MIST), Regulates Macrophage Activation During Obesity. |
