SMARCA5 Polyclonal antibody

• 

  WB analysis of HeLa using 13066-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13066-1-AP (SMARCA5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13066-1-AP

  WB result of SMARCA5 antibody (13066-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA5 transfected HEK-293 cells.

• 

  IHC staining of human stomach using 13066-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13066-1-AP (SMARCA5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13066-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMARCA5 antibody (13066-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

SMARCA5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13066-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:3.6.4.-, hISWI, hSNF2H, ISWI, SNF2H

---

Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

13066-1-AP targets SMARCA5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3759

  Product name: Recombinant human SMARCA5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 703-1052 aa of BC023144

  Sequence: FTMDTESSVYNFEGEDYREKQKIAFTEWIEPPKRERKANYAVDAYFREALRVSEPKAPKAPRPPKQPNVQDFQFFPPRLFELLEKEILFYRKTIGYKVPRNPELPNAAQAQKEEQLKIDEAESLNDEELEEKEKLLTQGFTNWNKRDFNQFIKANEKWGRDDIENIAREVEGKTPEEVIEYSAVFWERCNELQDIEKIMAQIERGEARIQRRISIKKALDTKIGRYKAPFHQLRISYGTNKGKNYTEEEDRFLICMLHKLGFDKENVYDELRQCIRNSPQFRFDWFLKSRTAMELQRRCNTLITLIERENMELEEKEKAEKKKRGPKPSTQKRKMDGAPDGRGRKKKLKL

• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
• Calculated Molecular Weight: 1052 aa, 122 kDa
• Observed Molecular Weight: 122 kDa
• GenBank Accession Number: BC023144
• Gene Symbol: SMARCA5
• Gene ID (NCBI): 8467
• RRID: AB_2191854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60264
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCA5, also named as SNF2H or WCRF135, is a 1052 amino acid protein, which localizes in nucleus and is overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. SMARCA5 is one of the most conserved chromatin remodeling factors. It exists in a variety of oligosubunit complexes that move DNA with respect to the histone octamer to generate regularly spaced nucleosomal arrays. Smarca5 interacts with different accessory proteins and represents a molecular motor for DNA replication, repair, and transcription.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Exp Med; Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.; Authors - Zhe Chen
human	WB	Nucleic Acids Res; SIRT6 coordinates with CHD4 to promote chromatin relaxation and DNA repair.; Authors - Tianyun Hou ; KD Validated
human	WB,IP,IF	Cell Death Dis; USP3 promotes DNA damage response and chemotherapy resistance through stabilizing and deubiquitinating SMARCA5 in prostate cancer; Authors - Sheng Li
human	WB	J Cell Biol; Stress granules regulate stress-induced paraspeckle assembly.; Authors - Haiyan An
		Arterioscler Thromb Vasc Biol; Novel Long Noncoding RNA, Macrophage Inflammation-Suppressing Transcript (MIST), Regulates Macrophage Activation During Obesity.; Authors - Kenneth Stapleton