SMARCA5 Recombinant antibody

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  WB analysis using 85019-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85019-3-RR (SMARCA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85019-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMARCA5 antibody (85019-3-RR, Clone: 242512A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 85019-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMARCA5 Recombinant antibody (85019-3-RR, Clone:242512A8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

SMARCA5 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 85019-3-RR

Clone No.242512A8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

242512A8, EC:3.6.4.-, hISWI, hSNF2H, ISWI

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HCT 116 cells, HepG2 cells, MCF-7 cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85019-3-RR targets SMARCA5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SMARCA5 fusion protein Ag3759
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
• Calculated Molecular Weight: 1052 aa, 122 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC023144
• Gene Symbol: SMARCA5
• Gene ID (NCBI): 8467
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60264
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCA5, also named as SNF2H or WCRF135, is a 1052 amino acid protein, which localizes in nucleus and is overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. SMARCA5 is one of the most conserved chromatin remodeling factors. It exists in a variety of oligosubunit complexes that move DNA with respect to the histone octamer to generate regularly spaced nucleosomal arrays. Smarca5 interacts with different accessory proteins and represents a molecular motor for DNA replication, repair, and transcription.