Tested Applications
| Positive WB detected in | HepG2 cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lymphoma tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20654-1-AP targets SMARCB1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | NM_003073 |
| Gene Symbol | SMARCB1 |
| Gene ID (NCBI) | 6598 |
| RRID | AB_10695761 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12824 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMARCB1, also named as BAF47, INI1 and SNF5L1, belongs to the SNF5 family. It is a core component of the BAF (hSWI/SNF) complex. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. SMARCB1 stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. It is involved in activation of CSF1 promoter. SMARCB1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. SMARCB1 plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Defects in SMARCB1 are a cause of rhabdoid tumor (RDT) which also known as malignant rhabdoid tumor (MRT). Defects in SMARCB1 are a cause of schwannomatosis. The antibody is specific to SMARCB1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SMARCB1 antibody 20654-1-AP | Download protocol |
| IP protocol for SMARCB1 antibody 20654-1-AP | Download protocol |
| WB protocol for SMARCB1 antibody 20654-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-SMARCB1 (SNF5), catalog number 20654-1-AP, has 9 total citations published in journals including Nature Cell Biology, PLoS Pathogens, Scientific Reports, Journal of Pathology, Translational Oncology, Frontiers in Cell and Developmental Biology, Cellular and Molecular Biology Letters, Acta Biochimica et Biophysica Sinica, and Bioengineered. Research fields span cardiac biology, triple-negative breast cancer, atherogenesis, melanoma, viral infection, chordoma, Coffin-Siris syndrome, non-small cell lung cancer, and digestive system malignancies.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function. | ||
Cell Mol Biol Lett A novel SWI/SNF complex promotes triple-negative breast cancer progression | ||
Front Cell Dev Biol Upregulation of Neogenin-1 by a CREB1-BAF47 Complex in Vascular Endothelial Cells is Implicated in Atherogenesis.
| ||
Transl Oncol SNF5, a core subunit of SWI/SNF complex, regulates melanoma cancer cell growth, metastasis, and immune escape in response to matrix stiffness. | ||
Sci Rep SMARCB1 orchestrates cellular plasticity and oncogenic pathways in typical and chondroid chordomas | ||
PLoS Pathog Reoviruses hijack the SMARCB1-MYC transcriptional regulation complex to activate autophagy for persistent viral infection in leafhopper vector. |
