Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue, Jurkat cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human lymphoma tissue, human stomach cancer tissue, rat brain tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10998-2-AP targets SMARCD1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1453 Product name: Recombinant human SMARCD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 126-476 aa of BC009368 Sequence: RLDIQEALKRPIKQKRKLRIFISNTFNPAKSDAEDGEGTVASWELRVEGRLLEDSALSKYDATKQKRKFSSFFKSLVIELDKDLYGPDNHLVEWHRTATTQETDGFQVKRPGDVNVRCTVLLMLDYQPPQFKLDPRLARLLGIHTQTRPVIIQALWQYIKTHKLQDPHEREFVICDKYLQQIFESQRMKFSEIPQRLHALLMPPEPIIINHVISVDPNDQKKTACYDIDVEVDDTLKTQMNSFLLSTASQQEIATLDNKIHETIETINQLKTQREFMLSFARDPQGFINDWLQSQCRDLKTMTDVVGNPEEERRAEFYFQPWAQEAVCRYFYSKVQQRRQELEQALGIRNT Predict reactive species |
| Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC009368 |
| Gene Symbol | SMARCD1 |
| Gene ID (NCBI) | 6602 |
| RRID | AB_2192133 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96GM5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMARCD1 belongs to the nuclear SWI/SNF chromatin remodeling complex, which regulates transcription and interacts with nucleolin in the nucleus. In addition, it's a member of the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). It possesses a SWIB domain which is homologous to and shares a common fold with the p53-binding domain of MDM2, a cell cycle regulator which interacts with NPM1. SMARCD1 has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). And it can mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. This antibody may have across reaction with SMARCD2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SMARCD1 antibody 10998-2-AP | Download protocol |
| IHC protocol for SMARCD1 antibody 10998-2-AP | Download protocol |
| IP protocol for SMARCD1 antibody 10998-2-AP | Download protocol |
| WB protocol for SMARCD1 antibody 10998-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SMARCD1 Polyclonal antibody (Cat# 10998-2-AP) has 11 citations spanning journals including Nature Communications, Nucleic Acids Research, Journal of Clinical Investigation, Cancer Letters, Biochemical Pharmacology, iScience, Journal of Biological Chemistry, and others. Research fields cover chromatin remodeling, myogenic gene transcription, renal cancer, ovarian carcinoma, liver transcription, diabetic cardiac fibrosis, Williams syndrome, melanoma, hepatocyte senescence, and histone variant dynamics.
| Species | Application | Title |
|---|---|---|
Nat Commun BAF60a deficiency uncouples chromatin accessibility and cold sensitivity from white fat browning.
| ||
Nucleic Acids Res HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription. | ||
J Clin Invest Genome-wide CRISPR screen identifies a cytokine-enhancer circuit driving HIF-2α activation in renal cancer.
| ||
Cancer Lett MicroRNA-490-3P targets CDK1 and inhibits ovarian epithelial carcinoma tumorigenesis and progression. | ||
Biochem Pharmacol ncBAF enhances PXR-mediated transcriptional activation in the human and mouse liver | ||
J Cell Mol Med DLEU1 contributes to ovarian carcinoma tumourigenesis and development by interacting with miR-490-3p and altering CDK1 expression. |
Reviews
What customers say
The single review for this antibody is a 5-star rating. The user applied it for Western Blot at a 1:1000 dilution, specifically in microwestern arrays. They reported obtaining precise and biologically relevant results, with clean, smear-free blots. Overall, the feedback highlights strong specificity and clean signal performance in a sensitive array-based format.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marian (Verified Customer) (11-13-2019) | We used SMARCD1 Antibody to perform microwestern arrays. We obtained very precise and biologically relevant results. The blots were clean and smear-free.
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