SMC1A Polyclonal antibody

SMC1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21695-1-AP

Synonyms

CDLS2, DXS423E, KIAA0178, SB1.8, SMC 1 alpha, SMC 1A, SMC protein 1A, SMC1, SMC1A, SMC1alpha, SMC1L1, SMCB



Tested Applications

Positive WB detected inA431 cells, HeLa cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21695-1-AP targets SMC1A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMC1A fusion protein Ag16286
Full Name structural maintenance of chromosomes 1A
Calculated Molecular Weight 1233 aa, 143 kDa
Observed Molecular Weight 143-150 kDa
GenBank Accession NumberBC112127
Gene Symbol SMC1A
Gene ID (NCBI) 8243
RRIDAB_2878904
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Structural maintenance of chromosomes protein 1A (SMC1A) is involved in chromosome cohesion during cell cycle and in DNA repair. It is a central component of cohesin complex which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Protocols

Product Specific Protocols
WB protocol for SMC1A antibody 21695-1-APDownload protocol
IHC protocol for SMC1A antibody 21695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Immunol

Interactome of the Autoimmune Risk Protein ANKRD55.

Authors - Nerea Ugidos
humanWB,IHC

Cancer Med

PIK3R3 is upregulated in liver cancer and activates Akt signaling to control cancer growth by regulation of CDKN1C and SMC1A

Authors - Weidong Lin
humanWB

Front Genet

In-Frame Variants in STAG3 Gene Cause Premature Ovarian Insufficiency.

Authors - Wen-Juan Xiao
human,mouseIF

Clin Transl Med

Sulfotransferase SULT2B1 facilitates colon cancer metastasis by promoting SCD1-mediated lipid metabolism

Authors - Gang Che