SMC3 Polyclonal antibody

SMC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14185-1-AP

Synonyms

Chromosome-associated polypeptide, CDLS3, BMH, Basement membrane-associated chondroitin proteoglycan, Bamacan

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Validation Data Gallery

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  WB analysis of HeLa using 14185-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14185-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14185-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 14185-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 14185-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14185-1-AP (SMC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14185-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14185-1-AP (SMC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, mouse brain tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• CoIP: See 1 publications below

Product Information

14185-1-AP targets SMC3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMC3 fusion protein Ag5440
• Full Name: structural maintenance of chromosomes 3
• Calculated Molecular Weight: 142 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC047324
• Gene Symbol: SMC3
• Gene ID (NCBI): 9126
• RRID: AB_2192625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	CoIP	Int J Mol Sci; APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.; Authors - Marianna Naki
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali
mouse	WB	Cell Mol Life Sci; RetSat stabilizes mitotic chromosome segregation in pluripotent stem cells; Authors - Wanzhi Cai
human	WB	Heliyon; ZNF521 promotes acute myeloid leukemogenesis by suppressing the expression and acetylation of SMC3; Authors - Rong Qin