SMC4 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• SMC4 Polyclonal antibody (24758-1-AP)

• Featured Product
• KD/KO Validated

SMC4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24758-1-AP Copied!

Publications (7)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CAP C, CAPC, Chromosome-associated polypeptide C, hCAP C, hCAPC

• 

  WB analysis using 24758-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24758-1-AP (SMC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24758-1-AP (SMC4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 24758-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMC4 antibody (24758-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for SMC4 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, C6 cells
• Positive IHC detected in: human colon tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24758-1-AP targets SMC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19156

  Product name: Recombinant human SMC4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 992-1288 aa of BC106033

  Sequence: SVLIHNSDEHKDIQSCTVEVHFQKIIDKEGDDYEVIPNSNFYVSRTACRDNTSVYHISGKKKTFKDVGNLLRSHGIDLDHNRFLILQGEVEQIAMMKPKGQTEHDEGMLEYLEDIIGCGRLNEPIKVLCRRVEILNEHRGEKLNRVKMVEKEKDALEGEKNIAIEFLTLENEIFRKKNHVCQYYIYELQKRIAEMETQKEKIHEDTKEINEKSNILSNEMKAKNKDVKDTEKKLNKITKFIEENKEKFTQLDLEDVQVREKLKHATSKAKKLEKQLQKDKEKVEEFKSIPAKSNNII

• Full Name: structural maintenance of chromosomes 4
• Calculated Molecular Weight: 1288 aa, 147 kDa
• Observed Molecular Weight: 147-150 kDa
• GenBank Accession Number: BC106033
• Gene Symbol: SMC4
• Gene ID (NCBI): 10051
• RRID: AB_2879707
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NTJ3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The calculated molecular weight of SMC4 is 147 kDa, but modified SMC4 is about 160 kDa.

Publications

What published studies show

The anti-SMC4 antibody (Cat# 24758-1-AP) has 7 total citations. Citations appear in Cell Metabolism, Journal of Translational Medicine, Scientific Reports, Frontiers in Oncology, Human Molecular Genetics, International Journal of Immunopathology and Pharmacology, and Gene. Research fields span colorectal cancer, cervical cancer progression, glioma metastasis, colon adenocarcinoma diagnosis/prognosis, condensin complex biology in Bloom syndrome, centrosome proteomics, and skeletal muscle hypertrophy.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IHC,IF	Cell Metab; The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity; Authors - Xuedan Sun ; KO Validated
human	WB	J Transl Med; SMC4/SMAD3/NF-κB axis drives cervical cancer progression and radioresistance via DNA damage repair and immune modulation.; Authors - Haixia Wu ; KD Validated
		Sci Rep; Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes.; Authors - Johan Busselez
human	WB,IF	Front Oncol; NFIA-dependent upregulation of SMC4 promotes metastasis and metabolic reprogramming in glioma.; Authors - Guoyin Li ; KD Validated
human	WB	Hum Mol Genet; Single-cell transcription profiles in Bloom syndrome patients link BLM deficiency with altered condensin complex expression signatures.; Authors - Ipek Ilgin Gönenc
human	WB,IHC	Int J Immunopathol Pharmacol; SMC4 serves as a potential marker for the diagnosis and prognosis of colon adenocarcinoma; Authors - Dawei Yang