SMC4 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMC4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 24758-1-AP

Synonyms

hCAP-C, hCAPC, hCAP C, Chromosome-associated polypeptide C, CAPC

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Validation Data Gallery

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  WB analysis of MCF-7 using 24758-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24758-1-AP (SMC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24758-1-AP (SMC4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 24758-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMC4 antibody (24758-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human colon tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

24758-1-AP targets SMC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMC4 fusion protein Ag19156
• Full Name: structural maintenance of chromosomes 4
• Calculated Molecular Weight: 1288 aa, 147 kDa
• Observed Molecular Weight: 147-150 kDa
• GenBank Accession Number: BC106033
• Gene Symbol: SMC4
• Gene ID (NCBI): 10051
• RRID: AB_2879707
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The calculated molecular weight of SMC4 is 147 kDa, but modified SMC4 is about 160 kDa.

Publications

Species	Application	Title
human	WB	Hum Mol Genet; Single-cell transcription profiles in Bloom syndrome patients link BLM deficiency with altered condensin complex expression signatures.; Authors - Ipek Ilgin Gönenc
rat	IHC	Gene; Transcriptional profiling of rat skeletal muscle hypertrophy under restriction of blood flow.; Authors - Shouyu Xu
		Sci Rep; Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes.; Authors - Johan Busselez
human,mouse	WB,IHC,IF	Cell Metab; The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity; Authors - Xuedan Sun ; KO Validated