SMC5 Polyclonal antibody

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SMC5 Polyclonal Antibody for WB, ELISA

Cat No. 14178-1-AP Copied!

Publications (4)

Reviews (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

SMC5L1, SMC-5, SMC protein 5, SMC 5, KIAA0594

• 

  WB analysis of HeLa using 14178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14178-1-AP (SMC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 14178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14178-1-AP (SMC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14178-1-AP targets SMC5 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5385

  Product name: Recombinant human SMC5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 748-1101 aa of BC038225

  Sequence: KLVTELTNLIKICTSLHIQKVDLILQNTTVISEKNKLESDYMAASSQLRLTEQHFIELDENRQRLLQKCKELMKRARQVCNLGAEQTLPQEYQTQVPTIPNGHNSSLPMVFQDLPNTLDEIDALLTEERSRASCFTGLNPTIVQEYTKREEEIEQLTEELKGKKVELDQYRENISQVKERWLNPLKELVEKINEKFSNFFSSMQCAGEVDLHTENEEDYDKYGIRIRVKFRSSTQLHELTPHHQSGGERSVSTMLYLMALQELNRCPFRVVDEINQGMDPINERRVFEMVVNTACKENTSQYFFITPKLLQNLPYSEKMTVLFVYNGPHMLEPNTWNLKAFQRRRRRITFTQPS

• Full Name: structural maintenance of chromosomes 5
• Calculated Molecular Weight: 1101 aa, 129 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC038225
• Gene Symbol: SMC5
• Gene ID (NCBI): 23137
• RRID: AB_2192775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IY18
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The SMC5 Polyclonal antibody (Cat# 14178-1-AP) has 4 citations published in Cell Reports, Stem Cell Reports, mSystems, and Journal of Virology. Research fields span viral replication and host–virus interactions (EBV, adenovirus, DNA viruses), SMC5/6 cohesin complex biology, and cancer-associated copy number variations in human embryonic stem cells.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Rep; Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.; Authors - Stephanie Pei Tung Yiu
		Stem Cell Reports; Early replication fragile sites are associated with cancer-related CNVs and SNVs in human embryonic stem cells.; Authors - Yu-Ping Dong
human	WB	mSystems; Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes; Authors - Joseph M. Dybas ; KD Validated
human	IF	J Virol; PJA1 Coordinates with the SMC5/6 Complex to Restrict DNA Viruses and Episomal Genes through Interferon-independent Manner.; Authors - Wei Xu

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

The single review for this antibody reports a fair result for SMC5 detection by Western Blot. The user tested it on HepG2 cells at a 1:1000 dilution and rated it 4 out of 5 stars, describing the performance as acceptable rather than exceptional.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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James (Verified Customer) (11-30-2022)

The detection of SMC5 by WB is fair with this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HepG2 cell

SMC5 Antibody Western Blot validation (1/1000 dilution) in HepG2 cell (Cat no:14178-1-AP)