SMCR7/MID49 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMCR7/MID49 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

54

Reviews (1)

Cat no : 16413-1-AP

Synonyms

MID49, MIEF2, SMCR7

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Validation Data Gallery

• 

  WB analysis using 16413-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 16413-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16413-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16413-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16413-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 16413-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16413-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 16413-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 16413-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HepG2 cells, HT-1080 cells
• Positive IHC detected in: human liver cancer tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 52 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

16413-1-AP targets SMCR7/MID49 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMCR7/MID49 fusion protein Ag9531
• Full Name: Smith-Magenis syndrome chromosome region, candidate 7
• Calculated Molecular Weight: 454 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC014973
• Gene Symbol: MID49
• Gene ID (NCBI): 125170
• RRID: AB_2714217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMCR7, also named as MID49, is a 49 kDa mitochondrial outer membrane protein which regulates mitochondrial morphology. SMCR7 can directly recruit the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. SMCR7 has two isoforms.

Publications

Species	Application	Title
human		Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KO Validated
human	WB	Autophagy; Loss of MIEF1/MiD51 confers susceptibility to BAX-mediated cell death and PINK1-PRKN-dependent mitophagy.; Authors - Hongxu Xian ; KD Validated
mouse	WB,IF	J Cell Biol; The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission.; Authors - Ruchika Anand
human	WB	EMBO J; FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.; Authors - Wenxian Wu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chun (Verified Customer) (07-03-2019)

This antibody is very good.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: HEK293 and HeLa cells

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