SMEK1 Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• SMEK1 Polyclonal antibody, PBS Only (26153-1-PBS)

• 

  WB analysis using 26153-1-AP (same clone as 26153-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26153-1-AP (SMEK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26153-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 26153-1-AP (same clone as 26153-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26153-1-AP (SMEK1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26153-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 26153-1-AP (same clone as 26153-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SMEK1 antibody (26153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 26153-1-PBS in a different storage buffer formulation.

SMEK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 26153-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KIAA2010, PP4R3A, PPP4R3A

---

Product Information

26153-1-PBS targets SMEK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5070

  Product name: Recombinant human SMEK1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 246-594 aa of BC038932

  Sequence: LTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS

• Full Name: SMEK homolog 1, suppressor of mek1 (Dictyostelium)
• Calculated Molecular Weight: 833 aa, 95 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC038932
• Gene Symbol: SMEK1
• Gene ID (NCBI): 55671
• RRID: AB_3742171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6IN85
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PPP4R3A also known as SMEK1, is the regulatory subunit of serine/threonine-protein phosphatase 4. SMEK1 regulates the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA DSB repair(PMID: 18614045).