Tested Applications
| Positive WB detected in | mouse brain tissue, mouse cerebellum tissue |
| Positive IHC detected in | human brain tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14609-1-AP targets SMPD1,ASM in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6180 Product name: Recombinant human SMPD1,ASM protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 47-364 aa of BC041164 Sequence: LSDSRVLWAPAEAHPLSPQGHPARLHRIVPRLRDVFGWGNLTCPICKGLFTAINLGLKKEPNVARVGSVAIKLCNLLKIAPPAVCQSIVHLFEDDMVEVWRRSVLSPSEACGLLLGSTCGHWDIFSSWNISLPTVPKPPPKPPSPPAPGAPVSRILFLTDLHWDHDYLEGTDPDCADPLCCRRGSGLPPASRPGAGYWGEYSKCDLPLRTLESLLSGLGPAGPFDMVYWTGDIPAHDVWHQTRQDQLRALTTVTALVRKFLGPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRWLYEAMAKAWEPWLPAEALRTLRCI Predict reactive species |
| Full Name | sphingomyelin phosphodiesterase 1, acid lysosomal |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 60 kDa, 70-75 kDa |
| GenBank Accession Number | BC041164 |
| Gene Symbol | SMPD1 |
| Gene ID (NCBI) | 6609 |
| RRID | AB_2878068 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17405 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMPD1(Sphingomyelin phosphodiesterase) is also named as ASM and belongs to the acid sphingomyelinase family. It is an important enzyme in sphingolipid metabolism and plays key roles in apoptosis, immunity, development, and cancer. The deficient activity of this enzyme results in Types A and B Niemann-Pick disease (NPD). This protein has 4 isoforms produced by alternative splicing. SMPD1 can produce a 60 kDa peptide with its signal peptide missing.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SMPD1,ASM antibody 14609-1-AP | Download protocol |
| WB protocol for SMPD1,ASM antibody 14609-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SMPD1/ASM Polyclonal antibody (Cat# 14609-1-AP) has accumulated 31 citations in journals including Nature Communications, Cell Host & Microbe, Cancer Research, EMBO Journal, Molecular Therapy, Journal of Biological Chemistry, and Acta Neuropathologica Communications. Research fields encompass sphingolipid metabolism, lipid homeostasis, extracellular vesicles, cancer (hepatocellular, pancreatic, colorectal, breast, melanoma, osteosarcoma), neurodegeneration (Alzheimer's, Parkinson's, spinal muscular atrophy), diabetes, atherosclerosis, and immune responses.
| Species | Application | Title |
|---|---|---|
Cell Host Microbe The β2Integrin Mac-1 Induces Protective LC3-Associated Phagocytosis of Listeria monocytogenes.
| ||
J Extracell Vesicles Ceramide-mediated orchestration of oxidative stress response through filopodia-derived small extracellular vesicles | ||
Nat Commun Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodents | ||
Adv Sci (Weinh) AXL Promotes Ischemic Myelin Repair Through Alleviating Myelin Debris Deposition and Lipid Droplets Accumulation. | ||
Adv Sci (Weinh) Nicotine Reprograms Aging-Related Metabolism and Protects Against Motor Decline in Mice |
Reviews
What customers say
The single review for this antibody is a 5-star rating from an IHC application on mouse pancreas tissue at a 1:200 dilution. The reviewer expressed satisfaction with the product's performance and suggested that the company showcase such positive results on its website to build confidence among potential users. Overall, the feedback is favorable, indicating reliable staining performance in immunohistochemistry.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alice (Verified Customer) (05-11-2021) | Good Ab should show good result in your website to convince people to use it.
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