Tested Applications
| Positive WB detected in | K-562 cells, human heart tissue, Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15979-1-AP targets Spermine synthase in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8767 Product name: Recombinant human SMS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-366 aa of BC009898 Sequence: MAAARHSTLDFMLGAKADGETILKGLQSIFQEQGMAESVHTWQDHGYLATYTNKNGSFANLRIYPHGLVLLDLQSYDGDAQGKEEIDSILNKVEERMKELSQDSTGRVKRLPPIVRGGAIDRYWPTADGRLVEYDIDEVVYDEDSPYQNIKILHSKQFGNILILSGDVNLAESDLAYTRAIMGSGKEDYTGKDVLILGGGDGGILCEIVKLKPKMVTMVEIDQMVIDGCKKYMRKTCGDVLDNLKGDCYQVLIEDCIPVLKRYAKEGREFDYVINDLTAVPISTSPEEDSTWEFLRLILDLSMKVLKQDGKYFTQGNCVNLTEALSLYEEQLGRLYCPVEFSKEIVCVPSYLELWVFYTVWKKAKP Predict reactive species |
| Full Name | spermine synthase |
| Calculated Molecular Weight | 366 aa, 41 kDa |
| Observed Molecular Weight | 41 kDa, 60 kDa |
| GenBank Accession Number | BC009898 |
| Gene Symbol | SMS |
| Gene ID (NCBI) | 6611 |
| RRID | AB_2270820 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52788 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Spermine synthase antibody 15979-1-AP | Download protocol |
| IF protocol for Spermine synthase antibody 15979-1-AP | Download protocol |
| WB protocol for Spermine synthase antibody 15979-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Spermine synthase Polyclonal antibody (Cat# 15979-1-AP) has 14 citations across journals including Cancer Letters, Phytomedicine, Alzheimer's Research & Therapy, Cells, International Journal of Molecular Sciences, Frontiers in Pharmacology, Scientific Reports, Proteomics, Bone, International Journal of Medical Sciences, Biology of Reproduction, Amino Acids, Journal of Proteomics, and Heliyon. Research fields span pancreatic cancer, ulcerative colitis, tauopathy, cellular senescence, glioma, lung adenocarcinoma, colorectal cancer, macrophage polarization, osteoblast biology, reproductive biology, muscle proteomics, and liver aging.
| Species | Application | Title |
|---|---|---|
Cancer Lett Multi-omics analysis of polyamine metabolism implicates NT5E/CD73 in the progression of pancreatic cancer | ||
Phytomedicine Oleanolic acid alleviates ulcerative colitis by remodeling Th17 cells via targeting HSP90β-ODC1 pathway-mediated polyamine synthesis. | ||
Alzheimers Res Ther Spermidine/spermine-N1-acetyltransferase ablation impacts tauopathy-induced polyamine stress response. | ||
Cells Magnesium Supplementation Attenuates Ultraviolet-B-Induced Damage Mediated through Elevation of Polyamine Production in Human HaCaT Keratinocytes. | ||
Front Pharmacol The RET inhibitor pralsetinib suppresses TMZ-resistant glioma growth by regulating spermine production. |
Reviews
What customers say
The single review for this antibody reports use in Western Blot on mouse liver lysate at a 1:500 dilution. The user rated it 3 out of 5, noting that while the antibody does work in WB, the signal produced is very weak. Overall, the feedback suggests the product is functional but underperforms in terms of signal strength for this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (03-28-2025) | This antibody is working in WB with mouse liver lysate. However the signal is very weak.
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