Spermine synthase Polyclonal antibody

Spermine synthase Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 15979-1-AP

Synonyms

MRSR, SMS, Spermine synthase, SPMSY, SpS

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Validation Data Gallery

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  WB analysis using 15979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15979-1-AP (Spermine synthase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15979-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15979-1-AP (Spermine synthase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells, human heart tissue, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 2 publications below

Product Information

15979-1-AP targets Spermine synthase in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Spermine synthase fusion protein Ag8767
• Full Name: spermine synthase
• Calculated Molecular Weight: 366 aa, 41 kDa
• Observed Molecular Weight: 41 kDa, 60 kDa
• GenBank Accession Number: BC009898
• Gene Symbol: SMS
• Gene ID (NCBI): 6611
• RRID: AB_2270820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC,WB	Sci Rep; Predicting lung adenocarcinoma prognosis, immune escape, and pharmacomic profile from arginine and proline-related genes; Authors - Ziqiang Wang
human	WB	Cells; Magnesium Supplementation Attenuates Ultraviolet-B-Induced Damage Mediated through Elevation of Polyamine Production in Human HaCaT Keratinocytes.; Authors - Shokoku Shu
human	WB	Proteomics; Changes in the proteomic profile during the differential polarization status of the human monocyte-derived macrophage THP-1 cell line.; Authors - Fan Zhang
mouse	WB	J Proteomics; Proteomic analysis revealed different responses to hypergravity of soleus and extensor digitorum longus muscles in mice.; Authors - Takashi Ohira
	IHC	Biol Reprod; Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period.; Authors - Katherine M Halloran
mouse	WB	Alzheimers Res Ther; Spermidine/spermine-N1-acetyltransferase ablation impacts tauopathy-induced polyamine stress response.; Authors - Leslie A Sandusky-Beltran