SMU1 Polyclonal antibody

SMU1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15511-1-AP

Synonyms

WD40 repeat-containing protein SMU1, Smu-1 suppressor of mec-8 and unc-52 protein homolog, SMU 1, SAP57, fSAP57

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Validation Data Gallery

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  WB analysis of mouse liver using 15511-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15511-1-AP (SMU1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15511-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15511-1-AP (SMU1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 15511-1-AP

  Immunofluorescent analysis of HepG2 cells, using SMU1 antibody 15511-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse liver tissue, K-562 cells
• Positive IHC detected in: human kidney tissue, human brain tissue, human hepatocirrhosis tissue, human ovary tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15511-1-AP targets SMU1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMU1 fusion protein Ag7688
• Full Name: smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans)
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC002876
• Gene Symbol: SMU1
• Gene ID (NCBI): 55234
• RRID: AB_2270843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sum1, belongs to the WD repeat SMU1 family. Human SMU1 is a component of the spliceosome fSAP57(PMID: 15254247). It is possible that the mutation in Smu1 affects spliceosome function, resulting in generation of improper splice variants, which could explain the phenotypes of tsTM18 cells. Some results suggest that Smu1 participates in at least two steps of splicing: splice-site choice and control of accuracy(PMID: 17105761). This antibody is a rabbit polyclonal antibody raised against a region of human REDD1 antigen.