Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human pancreas tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17594-1-AP targets SMYD4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11552 Product name: Recombinant human SMYD4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 201-578 aa of BC035077 Sequence: KDSLTESFPAALAKTLEDAALREENEQLSNASSSIGLCVDPLKGRCLVATKDILPGELLVQEDAFVSVLNPGELPPPHHGLDSKWDTRVTNGDLYCHRCLKHTLATVPCDGCSYAKYCSQECLQQVWELYHRTECPLGGLLLTLGVFCHIALRLTLLVGFEDVRKIITKLCDKISNKDICLPESNNQVKTLNYGLGESEKNGNIVETPIPGCDINGKYENNYNAVFNLLPHTENHSPEHKFLCALCVSALCRQLEAASLQAIPTERIVNSSQLKAAVTPELCPDVTIWGVAMLRHMLQLQCNAQAMTTIQHTGPKGSIVTDSRQVRLATGIFPVISLLNHSCSPNTSVSFISTVATIRASQRIRKGQEILHCYGPHKS Predict reactive species |
| Full Name | SET and MYND domain containing 4 |
| Calculated Molecular Weight | 804 aa, 89 kDa |
| Observed Molecular Weight | 89 kDa |
| GenBank Accession Number | BC035077 |
| Gene Symbol | SMYD4 |
| Gene ID (NCBI) | 114826 |
| RRID | AB_10694429 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IYR2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMYD4, also named as SET and MYND domain-containing protein 4, is a 804 amino acid protein, which contains 1 MYND-type zinc finger and 1 SET domain. SMYD4 belongs to the class V-like SAM-binding methyltransferase superfamily. SMYD4, as a potential tumor suppressor, plays a critical role in breast carcinogenesis at least partly through inhibiting the expression of Pdgfr-alpha, and could be a novel target for improving treatment of breast cancer. SMYD4 is a muscle-specific transcriptional modulator involved in development.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SMYD4 antibody 17594-1-AP | Download protocol |
| IHC protocol for SMYD4 antibody 17594-1-AP | Download protocol |
| WB protocol for SMYD4 antibody 17594-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SMYD4 Polyclonal antibody (Cat# 17594-1-AP) has a total of 4 citations. These publications appear in Breast Cancer Research, Cancer Science, PLoS Genetics, and Frontiers in Oncology. The associated research fields include breast cancer progression, hepatocellular carcinoma, epigenetic regulation of cardiac development, and primary malignant melanoma of the cervix.
| Species | Application | Title |
|---|---|---|
Breast Cancer Res SMYD4 promotes MYH9 ubiquitination through lysine monomethylation modification to inhibit breast cancer progression | ||
Cancer Sci SMYD4 monomethylates PRMT5 and forms a positive feedback loop to promote hepatocellular carcinoma progression | ||
PLoS Genet The roles of SMYD4 in epigenetic regulation of cardiac development in zebrafish.
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Front Oncol The genetic landscape of primary malignant melanoma of the cervix using integrated bioinformatics analysis and whole-exome sequencing.
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