SNAPC5 Polyclonal antibody

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  WB analysis of HeLa using 17272-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17272-1-AP (SNAPC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17272-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17272-1-AP (SNAPC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 17272-1-AP

  Immunofluorescent analysis of HeLa cells using 17272-1-AP (SNAPC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SNAPC5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17272-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

snRNA-activating protein complex subunit 5, snRNA-activating protein complex 19 kDa subunit, SNAPc subunit 5, SNAPC 5, SNAP19

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human testis tissue, human breast cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

17272-1-AP targets SNAPC5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11029

  Product name: Recombinant human SNAPC5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-68 aa of BC014315

  Sequence: MLSRLQELRKEEETLLRLKAALHDQLNRLKMLVHVDNEASINQTTLELSTKSHVTEEEEEEEEEESDS

• Full Name: small nuclear RNA activating complex, polypeptide 5, 19kDa
• Calculated Molecular Weight: 68aa,8 kDa; 98aa,11 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC014315
• Gene Symbol: SNAPC5
• Gene ID (NCBI): 10302
• RRID: AB_2878373
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75971
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNAPC5, also named as SNAP19, is snRNA-activating protein complex 19 kDa. It's a part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC,IF	Aging (Albany NY); Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression; Authors - Guoyang Zhang