SNAPC5 Polyclonal antibody

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• SNAPC5 Polyclonal antibody (17272-1-AP)

SNAPC5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17272-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

snRNA-activating protein complex subunit 5, snRNA-activating protein complex 19 kDa subunit, SNAPc subunit 5, SNAPC 5, SNAP19

• 

  WB analysis of HeLa using 17272-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17272-1-AP (SNAPC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 17272-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17272-1-AP (SNAPC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 17272-1-AP

  Immunofluorescent analysis of HeLa cells using 17272-1-AP (SNAPC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human testis tissue, human breast cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17272-1-AP targets SNAPC5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11029

  Product name: Recombinant human SNAPC5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-68 aa of BC014315

  Sequence: MLSRLQELRKEEETLLRLKAALHDQLNRLKMLVHVDNEASINQTTLELSTKSHVTEEEEEEEEEESDS

• Full Name: small nuclear RNA activating complex, polypeptide 5, 19kDa
• Calculated Molecular Weight: 68aa,8 kDa; 98aa,11 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC014315
• Gene Symbol: SNAPC5
• Gene ID (NCBI): 10302
• RRID: AB_2878373
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75971
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNAPC5, also named as SNAP19, is snRNA-activating protein complex 19 kDa. It's a part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes.

Publications

What published studies show

The SNAPC5 Polyclonal antibody (Cat# 17272-1-AP) has 1 citation. It was published in Aging (Albany NY) (2023), where it was used for WB, IHC, and IF in human samples. The study explored cancer dependency map genes and immune subtypes in colon cancer, specifically examining TIGD1's contribution to colon cancer progression. The research field is oncology, focusing on colon cancer molecular mechanisms.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC,IF	Aging (Albany NY); Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression; Authors - Guoyang Zhang