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  • KD/KO Validated

SND1 Polyclonal antibody

SND1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IHC, IF, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 10760-1-AP

Synonyms

100 kDa coactivator, EBNA2 coactivator p100, p100, p100 co activator, SND1, TDRD11



Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, Jurkat cells
Positive IHC detected inhuman liver tissue, human breast hyperplasia tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10760-1-AP targets SND1 in WB, RIP, IHC, IF, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SND1 fusion protein Ag1200
Full Name staphylococcal nuclease and tudor domain containing 1
Calculated molecular weight 101 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC017180
Gene symbol SND1
Gene ID (NCBI) 27044
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors. This is a rabbit polyclonal antibody raised against near the C-terminus of human SND1.

Protocols

Product Specific Protocols
WB protocol for SND1 antibody 10760-1-APDownload protocol
IHC protocol for SND1 antibody 10760-1-APDownload protocol
IF protocol for SND1 antibody 10760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Comp34 displays potent preclinical antitumor efficacy in triple-negative breast cancer via inhibition of NUDT3-AS4, a novel oncogenic long noncoding RNA.

Authors - Qiongyu Hao
WB

J Cell Physiol

The phosphorylation of Tudor-SN mediated by JNK is involved in the regulation of milk protein synthesis induced by prolactin in BMECs.

Authors - Sen Zhang
mouseWB,IHC

J Proteome Res

Novel breast cancer metastasis-associated proteins.

Authors - Ho Jiapei J
humanWB,ChIP,RIP

Elife

The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.

Authors - Belinda Baquero-Perez
  • KD Validated
humanRIP

Cell Res

Predicting dynamic cellular protein-RNA interactions by deep learning using in vivo RNA structures.

Authors - Lei Sun