SND1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SND1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 10760-1-AP

Synonyms

p100 co-activator, p100 co activator, p100, EC:3.1.31.1, EBNA2 coactivator p100

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Validation Data Gallery

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  WB analysis of Jurkat using 10760-1-AP

  WB result of SND1 antibody (10760-1-AP, 1:4000) with si-Control and si-SND1 transfected Jurkat cells.

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  WB analysis using 10760-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10760-1-AP (SND1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast hyperplasia using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10760-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10760-1-AP (SND1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, COLO 320 cells, HeLa cells
• Positive IHC detected in: human breast hyperplasia tissue, human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 2 publications below

Product Information

10760-1-AP targets SND1 in WB, IHC, IF/ICC, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SND1 fusion protein Ag1200
• Full Name: staphylococcal nuclease and tudor domain containing 1
• Calculated Molecular Weight: 101 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC017180
• Gene Symbol: SND1
• Gene ID (NCBI): 27044
• RRID: AB_2193410
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors. This is a rabbit polyclonal antibody raised against near the C-terminus of human SND1.

Publications

Species	Application	Title
human	IF	Cell; SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9; Authors - Nora Schmidt
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	RIP	Cell Res; Predicting dynamic cellular protein-RNA interactions by deep learning using in vivo RNA structures.; Authors - Lei Sun
human	WB,ChIP,RIP	Elife; The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.; Authors - Belinda Baquero-Perez ; KD Validated
mouse	WB,IHC	J Proteome Res; Novel breast cancer metastasis-associated proteins.; Authors - Ho Jiapei J
	WB	J Cell Physiol; The phosphorylation of Tudor-SN mediated by JNK is involved in the regulation of milk protein synthesis induced by prolactin in BMECs.; Authors - Sen Zhang