Validation Data Gallery
|Positive WB detected in||A549 cells, Jurkat cells, COLO 320 cells, HeLa cells|
|Positive IHC detected in||human breast hyperplasia tissue, human breast cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10760-1-AP targets SND1 in WB, RIP, IHC, IF, ChIP, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SND1 fusion protein Ag1200|
|Full Name||staphylococcal nuclease and tudor domain containing 1|
|Calculated molecular weight||101 kDa|
|Observed molecular weight||101 kDa|
|GenBank accession number||BC017180|
|Gene ID (NCBI)||27044|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors. This is a rabbit polyclonal antibody raised against near the C-terminus of human SND1.
SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9
Divergent transcriptional regulation of astrocyte reactivity across disorders.
Predicting dynamic cellular protein-RNA interactions by deep learning using in vivo RNA structures.
The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.
J Proteome Res
Novel breast cancer metastasis-associated proteins.
J Cell Physiol
The phosphorylation of Tudor-SN mediated by JNK is involved in the regulation of milk protein synthesis induced by prolactin in BMECs.