Tested Applications
| Positive WB detected in | A549 cells, Jurkat cells, COLO 320 cells, HeLa cells |
| Positive IHC detected in | human breast hyperplasia tissue, human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10760-1-AP targets SND1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1200 Product name: Recombinant human SND1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 548-911 aa of BC017180 Sequence: TCLITFLLAGIECPRGARNLPGLVQEGEPFSEEATLFTKELVLQREVEVEVESMDKAGNFIGWLHIDGANLSVLLVEHALSKVHFTAERSSYYKSLLSAEEAAKQKKEKVWAHYEEQPVEEVMPVLEEKERSASYKPVFVTEITDDLHFYVQDVETGTQLEKLMENMRNDIASHPPVEGSYAPRRGEFCIAKFVDGEWYRARVEKVESPAKIHVFYIDYGNREVLPSTRLGTLSPAFSTRVLPAQATEYAFAFIQVPQDDDARTDAVDSVVRDIQNTQCLLNVEHLSAGCPHVTLQFADSKGDVGLGLVKEGLVMVEVRKEKQFQKVITEYLNAQESAKSARLNLWRYGDFRADDADEFGYSR Predict reactive species |
| Full Name | staphylococcal nuclease and tudor domain containing 1 |
| Calculated Molecular Weight | 101 kDa |
| Observed Molecular Weight | 101 kDa |
| GenBank Accession Number | BC017180 |
| Gene Symbol | SND1 |
| Gene ID (NCBI) | 27044 |
| RRID | AB_2193410 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7KZF4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors. This is a rabbit polyclonal antibody raised against near the C-terminus of human SND1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SND1 antibody 10760-1-AP | Download protocol |
| IHC protocol for SND1 antibody 10760-1-AP | Download protocol |
| WB protocol for SND1 antibody 10760-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-SND1 (10760-1-AP) has 13 citations published in journals including Nature, Cell, Cell Research, Nature Cell Biology, Journal of Experimental & Clinical Cancer Research, Cell Death & Disease, Biochemical Pharmacology, Scientific Reports, Journal of Cellular Physiology, Journal of Proteome Research, Drug Metabolism and Disposition, Animal Biotechnology, and eLife. Research fields span neuroscience, viral replication, m6A RNA modification, pancreatic and breast cancer, glioma ferroptosis, lncRNA regulation, hepatocellular carcinoma, and mammary gland biology.
| Species | Application | Title |
|---|---|---|
Cell SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9 | ||
Cell Res Predicting dynamic cellular protein-RNA interactions by deep learning using in vivo RNA structures. | ||
Nat Cell Biol Psychological stress-induced ALKBH5 deficiency promotes tumour innervation and pancreatic cancer via extracellular vesicle transfer of RNA | ||
J Exp Clin Cancer Res Epigenetically silenced lncRNA SNAI3-AS1 promotes ferroptosis in glioma via perturbing the m6A-dependent recognition of Nrf2 mRNA mediated by SND1 | ||
Cell Death Dis Comp34 displays potent preclinical antitumor efficacy in triple-negative breast cancer via inhibition of NUDT3-AS4, a novel oncogenic long noncoding RNA. |
