SNF2L Polyclonal antibody

SNF2L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29461-1-AP

Synonyms

ATP dependent helicase SMARCA1, DKFZp686D1623, FLJ41547, ISWI, NURF140, SMARCA1, SNF2L, SNF2L1, SNF2LB, SWI, SWI2

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Validation Data Gallery

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  WB analysis using 29461-1-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 29461-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 29461-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat lung using 29461-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29461-1-AP (SNF2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 29461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29461-1-AP (SNF2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U-87 MG cells, U-251 cells, SH-SY5Y cells
• Positive IHC detected in: mouse lung tissue, rat lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29461-1-AP targets SNF2L in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNF2L fusion protein Ag30657
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1
• Calculated Molecular Weight: 123 kDa
• Observed Molecular Weight: 120-130 KDa
• GenBank Accession Number: NM_003069
• Gene Symbol: SMARCA1
• Gene ID (NCBI): 6594
• RRID: AB_2918310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNF2L, also named as SMARCA1, NURF140 and ISWI, encodes the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription (PMID: 28801535). SNF2L was shown to promote neurite outgrowth as well as regulate the human engrailed homeotic genes, important regulators of brain development (PMID: 15310751). The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells (PMID: 25462860). SNF2L has 2 isoforms with MW of 123 kDa and 121 kDa, and can be detected as 120-130 kDa.

Publications

Species	Application	Title
human	WB	Cell Death Differ; BAP18 facilitates CTCF-mediated chromatin accessible to regulate enhancer activity in breast cancer; Authors - Ge Sun