SNIP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SNIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 14950-1-AP

Synonyms

SNIP 1, Smad nuclear-interacting protein 1, FHA domain-containing protein SNIP1

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Validation Data Gallery

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  WB analysis using 14950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14950-1-AP (SNIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14950-1-AP

  IP result of anti-SNIP1 (IP:14950-1-AP, 4ug; Detection:14950-1-AP 1:1000) with HeLa cells lysate 2400ug.

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  IF Staining of HeLa using 14950-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNIP1 antibody (14950-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

14950-1-AP targets SNIP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNIP1 fusion protein Ag6772
• Full Name: Smad nuclear interacting protein 1
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC027040
• Gene Symbol: SNIP1
• Gene ID (NCBI): 79753
• RRID: AB_2286497
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Smad nuclear-interacting protein 1 (SNIP1) is a novel interacting factor of the Smad protein family that is expressed in different cell types and is involved in cell proliferation, apoptosis, and differentiation.

Publications

Species	Application	Title
human	WB,IHC,IP	Nat Commun; KMT5A-methylated SNIP1 promotes triple-negative breast cancer metastasis by activating YAP signaling.; Authors - Bo Yu ; KO Validated
human	WB	PeerJ; miR-29a-3p directly targets Smad nuclear interacting protein 1 and inhibits the migration and proliferation of cervical cancer HeLa cells.; Authors - Ying Chen ; KD Validated
human	WB,CoIP	bioRxiv; mRNA interactions with disordered regions control protein activity; Authors - Yang Luo
mouse	WB,IP	Nat Commun; SNIP1 and PRC2 coordinate cell fates of neural progenitors during brain development; Authors - Yurika Matsui
human	WB	Cell Death Differ; LncRNA BCAN-AS1 stabilizes c-Myc via N6-methyladenosine-mediated binding with SNIP1 to promote pancreatic cancer; Authors - Guandi Wu
human	WB	J Proteome Res; Quantitative Proteomics Reveal the Mechanism of MiR-138-5p Suppressing Cervical Cancer via Targeting ZNF385A; Authors - Qihang Peng