Tested Applications
| Positive WB detected in | U2OS cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14950-1-AP targets SNIP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6772 Product name: Recombinant human SNIP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-396 aa of BC027040 Sequence: MKAVKSERERGSRRRHRDGDVVLPAGVVVKQERLSPEVAPPAHRRPDHSGGSPSPPTSEPARSGHRGNRARGVSRSPPKKKNKASGRRSKSPRSKRNRSPHHSTVKVKQEREDHPRRGREDRQHREPSEQEHRRARNSDRDRHRGHSHQRRTSNERPGSGQGQGRDRDTQNLQAQEEEREFYNARRREHRQRNDVGGGGSESQELVPRPGGNNKEKEVPAKEKPSFELSGALLEDTNTFRGVVIKYSEPPEARIPKKRWRLYPFKNDEVLPVMYIHRQSAYLLGRHRRIADIPIDHPSCSKQHAVFQYRLVEYTRADGTVGRRVKPYIIDLGSGNGTFLNNKRIEPQRYYELKEKDVLKFGFSSREYVLLHESSDTSEIDRKDDEDEEEEEEVSDS Predict reactive species |
| Full Name | Smad nuclear interacting protein 1 |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 46-50 kDa |
| GenBank Accession Number | BC027040 |
| Gene Symbol | SNIP1 |
| Gene ID (NCBI) | 79753 |
| RRID | AB_2286497 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TAD8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Smad nuclear-interacting protein 1 (SNIP1) is a novel interacting factor of the Smad protein family that is expressed in different cell types and is involved in cell proliferation, apoptosis, and differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SNIP1 antibody 14950-1-AP | Download protocol |
| IP protocol for SNIP1 antibody 14950-1-AP | Download protocol |
| WB protocol for SNIP1 antibody 14950-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SNIP1 antibody (Cat# 14950-1-AP) has 10 total citations spanning journals including Nature, Nature Communications, Cell Death & Differentiation, iScience, Journal of Proteome Research, PeerJ, Thoracic Cancer, and bioRxiv. Research fields cover pre-mRNA splicing regulation, neural progenitor development, and multiple cancer types (triple-negative breast, pancreatic, ovarian, cervical, and lung), with applications primarily in Western blot and immunoprecipitation.
| Species | Application | Title |
|---|---|---|
Nat Commun KMT5A-methylated SNIP1 promotes triple-negative breast cancer metastasis by activating YAP signaling.
| ||
Nat Commun SNIP1 and PRC2 coordinate cell fates of neural progenitors during brain development | ||
Nat Commun Phosphorylation of SF3B1 by CDK11 orchestrates spliceosome activation via SNIP1-dependent RES complex recruitment. | ||
Cell Death Differ LncRNA BCAN-AS1 stabilizes c-Myc via N6-methyladenosine-mediated binding with SNIP1 to promote pancreatic cancer | ||
iScience Targeting SUMOylation in ovarian cancer: Sensitivity, resistance, and the role of MYC
|
