SNRNP200 Polyclonal antibody

SNRNP200 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 23875-1-AP

Synonyms

KIAA0788, HELIC2, EC:3.6.4.13, BRR2, ASCC3L1

---

Validation Data Gallery

• 

  WB analysis of HeLa using 23875-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 23875-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23875-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23875-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 23875-1-AP

  IP result of anti-SNRNP200 (IP:23875-1-AP, 4ug; Detection:23875-1-AP 1:1000) with HeLa cells lysate 4000ug.

• 

  IHC staining of human placenta using 23875-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 23875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 23875-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 23875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A431 using 23875-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human SNRNP200 (23875-1-AP) and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human placenta tissue, mouse bladder tissue, mouse testis tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IP: See 1 publications below

Product Information

23875-1-AP targets SNRNP200 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNRNP200 fusion protein Ag20748
• Full Name: small nuclear ribonucleoprotein 200kDa (U5)
• Calculated Molecular Weight: 494 aa, 57 kDa
• Observed Molecular Weight: 200-245 kDa
• GenBank Accession Number: BC001417
• Gene Symbol: SNRNP200
• Gene ID (NCBI): 23020
• RRID: AB_2879346
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNRNP200 (small nuclear ribonucleoprotein 200kDa (U5)), also known as HELIC2, ASCC3L1 or BRR2, is a 2,136 amino acid protein that localizes to the nucleus and contains two SEC63 domains, two helicase C-terminal domains and two helicase ATP-binding domains. Existing as multiple alternatively spliced isoforms, HELIC2 functions as an RNA helicase that is thought to promote specific RNA-RNA conformational changes which are important in the second step of RNA splicing. The gene encoding HELIC2 maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the chromosome 2-localized ABCA12 gene, while the lipid metabolic disorder sitosterolemia is associated with defects in the ABCG5 and ABCG8 genes, hich also map to chromosome 2. The observed molecular weight of SNRNP200 is 200-245kd.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou
human	IP	J Biol Chem; Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.; Authors - Zhe Chen
human	WB	Cell Rep; SART3 reads methylarginine-marked glycine- and arginine-rich motifs; Authors - Yalong Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Roy (Verified Customer) (06-15-2024)

Works fine by WB to detect SNRNP200 from HeLa extracts (1/1000 dilution, overnight incubation at 4°C)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa