SNRNP25 Polyclonal antibody

SNRNP25 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 19890-1-AP

Synonyms

C16orf33, Minus 99 protein, SNRNP25, U11/U12 25K, U11/U12 snRNP 25 kDa protein

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Validation Data Gallery

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  IHC staining of mouse cerebellum using 19890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19890-1-AP (SNRNP25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 19890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19890-1-AP (SNRNP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19890-1-AP targets SNRNP25 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNRNP25 fusion protein Ag13724
• Full Name: small nuclear ribonucleoprotein 25kDa (U11/U12)
• Calculated Molecular Weight: 132 aa, 15 kDa
• GenBank Accession Number: BC001381
• Gene Symbol: SNRNP25
• Gene ID (NCBI): 79622
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNRNP25, small nuclear ribonucleoprotein U11/U12 subunit 25, encodes a 25K protein that is a component of the U12-type spliceosome. Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99% of pre-mRNA introns. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. LRP1-SNRNP25 fusion gene promotes osteosarcoma cell invasion and migration (PMID: 26738504, PMID: 25300797).