SNRNP48 Polyclonal antibody

SNRNP48 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24297-1-AP

Synonyms

C6orf151, SNRNP48, U11/U12 48K, U11/U12 snRNP 48 kDa protein

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Validation Data Gallery

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  WB analysis of HEK-293 using 24297-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24297-1-AP (SNRNP48 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 24297-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24297-1-AP (SNRNP48 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 24297-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24297-1-AP (SNRNP48 Antibody) at dilution of 1:50.

• 

  IHC staining of human brain using 24297-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24297-1-AP (SNRNP48 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 24297-1-AP is SNRNP48 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNRNP48 fusion protein Ag18157
• Full Name: small nuclear ribonucleoprotein 48kDa (U11/U12)
• Calculated Molecular Weight: 339 aa, 40 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC114630
• Gene Symbol: SNRNP48
• Gene ID (NCBI): 154007
• RRID: AB_2879480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; Distinct functions for the paralogous RBM41 and U11/U12-65K proteins in the minor spliceosome; Authors - Antto J Norppa