SNRPB Polyclonal antibody

• Featured Product
• KD/KO Validated

SNRPB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16807-1-AP

Synonyms

COD, Sm B/B, Sm protein B/B, SmB/B, SmB/SmB', snRNP B, SNRPB, SNRPB1

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Validation Data Gallery

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  WB analysis using 16807-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16807-1-AP (SNRPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16807-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16807-1-AP (SNRPB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16807-1-AP (SNRPB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16807-1-AP (SNRPB Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16807-1-AP (SNRPB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16807-1-AP (SNRPB Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells
• Positive IHC detected in: human cervical cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Product Information

The immunogen of 16807-1-AP is SNRPB Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNRPB fusion protein Ag10298
• Full Name: small nuclear ribonucleoprotein polypeptides B and B1
• Calculated Molecular Weight: 234aa,25 kDa; 285aa,30 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC003530
• Gene Symbol: SNRPB
• Gene ID (NCBI): 6628
• RRID: AB_2878319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IP	Elife; Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation.; Authors - Maxim I Maron
human	IHC	Cell Death Dis; SNRPB promotes the tumorigenic potential of NSCLC in part by regulating RAB26.; Authors - Nianli Liu ; KO Validated
human	WB,IHC	Oncogene; The splicing factor SNRPB promotes ovarian cancer progression through regulating aberrant exon skipping of POLA1 and BRCA2; Authors - Yingwei Li ; KD Validated
human,mouse	WB,IHC	Aging (Albany NY); SNRPB promotes the progression of hepatocellular carcinoma via regulating cell cycle, oxidative stress, and ferroptosis; Authors - Xiaoyan Wang