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WB analysis using 67280-1-Ig (same clone as 67280-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67280-1-Ig (same clone as 67280-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67280-1-Ig (same clone as 67280-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67280-1-Ig (same clone as 67280-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67280-1-Ig (same clone as 67280-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.
IF Staining of SH-SY5Y using 67280-1-Ig (same clone as 67280-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SNRPN/SNRPB antibody (67280-1-Ig, Clone: 1H2F4 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP51682-1
Cytometric bead array sample test of MP51682-1, SNRPN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67280-1-PBS. Detection antibody: 67280-2-PBS.
Cytometric bead array standard curve of MP51682-1
Cytometric bead array standard curve of MP51682-1, SNRPN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67280-1-PBS. Detection antibody: 67280-2-PBS. Standard:Ag14030. Range: 0.781-100 ng/mL.

SNRPN/SNRPB Monoclonal antibody, PBS Only (Capture)

Name: SNRPN/SNRPB Monoclonal antibody, PBS Only (Capture)
Brand: Proteintech
SKU: 67280-1-PBS

SNRPN/SNRPB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67280-1-PBS

Clone No.1H2F4

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SNRPN, SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67280-1-PBS targets SNRPN/SNRPB as part of a matched antibody pair:

MP51682-1: 67280-1-PBS capture and 67280-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag14030 Product name: Recombinant human SNRPN protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-240 aa of BC000611 Sequence: MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNAKQPEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGVGRAAGRGVPAGVPIPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAVAATASIAGAPTQYPPGRGTPPPPVGRATPPPGIMAPPPGMRPPMGPPIGLPPARGTPIGMPPPGMRPPPPGIRGPPPPGMRPPRP Predict reactive species
Full Name	small nuclear ribonucleoprotein polypeptide N
Calculated Molecular Weight	240 aa, 25 kDa
Observed Molecular Weight	27-29 kDa
GenBank Accession Number	BC000611
Gene Symbol	SNRPN
Gene ID (NCBI)	6638
RRID	AB_2882548
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P63162
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SNRPN, or SNURF-SNRPN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide.The SNRPN gene is transcribed exclusively from the paternally inherited chromosome and shows highest expression in brain and heart. SNRPN may play a role in etiology of Prader-Willi syndrome. This antibody recognizes SNRPN and SNRPB due to high amino acid sequence homology.