SNRPN Monoclonal antibody

SNRPN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H2F4

Cat no : 67280-1-Ig

Synonyms

SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D, HCERN3

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Validation Data Gallery

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  WB analysis using 67280-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67280-1-Ig (SNRPN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67280-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67280-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67280-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67280-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 67280-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SNRPN antibody (67280-1-Ig, Clone: 1H2F4 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67280-1-Ig targets SNRPN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SNRPN fusion protein Ag14030
• Full Name: small nuclear ribonucleoprotein polypeptide N
• Calculated Molecular Weight: 240 aa, 25 kDa
• Observed Molecular Weight: 27-29 kDa
• GenBank Accession Number: BC000611
• Gene Symbol: SNRPN
• Gene ID (NCBI): 6638
• RRID: AB_2882548
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNRPN, or SNURF-SNRPN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide.The SNRPN gene is transcribed exclusively from the paternally inherited chromosome and shows highest expression in brain and heart. SNRPN may play a role in etiology of Prader-Willi syndrome.