SNW1 Polyclonal antibody

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  WB analysis of HepG2 using 25926-1-AP

  WB result of SNW1 antibody (25926-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNW1 transfected HepG2 cells.

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  WB analysis of MCF-7 using 25926-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25926-1-AP (SNW1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25926-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25926-1-AP (SNW1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 25926-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25926-1-AP (SNW1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 25926-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25926-1-AP (SNW1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 25926-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNW1 antibody (25926-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 25926-1-AP

  Immunofluorescent analysis of HepG2 cells using 25926-1-AP (SNW1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

SNW1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25926-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NCOA 62, SKIP, SKIIP, Ski-interacting protein, Nuclear receptor coactivator NCoA-62

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Tested Applications

• Positive WB detected in: MCF-7 cells, HepG2 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

25926-1-AP targets SNW1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23106

  Product name: Recombinant human SNW1 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 188-536 aa of BC108903

  Sequence: FNSGAKQRVIRMVEMQKDPMEPPRFKINKKIPRGPPSPPAPVMHSPSRKMTVKEQQEWKIPPCISNWKNAKGYTIPLDKRLAADGRGLQTVHINENFAKLAEALYIADRKAREAVEMRAQVERKMAQKEKEKHEEKLREMAQKARERRAGIKTHVEKEDGEARERDEIRHDRRKERQHDRNLSRAAPDKRSKLQRNENRDISEVIALGVPNPRTSNEVQYDQRLFNQSKGMDSGFAGGEDEIYNVYDQAWRGGKDMAQSIYRPSKNLDKDMYGDDLEARIKTNRFVPDKEFSGSDRRQRGREGPVQFEEDPFGLDKFLEEAKQHGGSKRPSDSSRPKEHEHEGKKRRKE

• Full Name: SNW domain containing 1
• Calculated Molecular Weight: 536 aa, 61 kDa
• Observed Molecular Weight: 62-69 kDa
• GenBank Accession Number: BC108903
• Gene Symbol: SNW1
• Gene ID (NCBI): 22938
• RRID: AB_2880298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13573
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou
human	WB	FEBS Lett; Split-BioID: a proximity biotinylation assay for dimerization-dependent protein interactions.; Authors - Sofie De Munter
human	WB	Microbes Infect; SNW1 interacts with IKKγ to positively regulate antiviral innate immune responses against influenza A virus infection.; Authors - Qiao Zhang ; KD Validated
mouse	WB	J Ovarian Res; Alteration of the N6-methyladenosine methylation landscape in a mouse model of polycystic ovary syndrome; Authors - Lingxiao Zou