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  • KD/KO Validated

SNX1 Polyclonal antibody

SNX1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10304-1-AP

Synonyms

HsT17379, SNX1, SNX1A, sorting nexin 1, Vps5



Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human kidney tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10304-1-AP targets SNX1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX1 fusion protein Ag0499
Full Name sorting nexin 1
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC000357
Gene Symbol SNX1
Gene ID (NCBI) 6642
RRIDAB_2192217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sorting nexin 1 (SNX1, synonyms: SNX1A, MGC8664, HsT17379) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. SNX1 also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members.

Protocols

Product Specific Protocols
WB protocol for SNX1 antibody 10304-1-APDownload protocol
IHC protocol for SNX1 antibody 10304-1-APDownload protocol
IP protocol for SNX1 antibody 10304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

Retromer dysfunction in amyotrophic lateral sclerosis.

Authors - Eduardo J Pérez-Torres
humanWB,IP

J Neurosci

A critical role for Sorting Nexin 1 in the trafficking of metabotropic glutamate receptors.

Authors - Rohan Sharma
  • KD Validated
mouseWB

FASEB J

Sorting nexin 1 loss results in increased oxidative stress and hypertension.

Authors - Jian Yang
  • KO Validated
mouseWB

J Biol Chem

PMID: 23152498

Authors - Villar Van Anthony M VA
  • KD Validated
mouseIF

Mol Biol Cell

Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks.

Authors - Gomez Timothy S TS
mouseIF

PLoS One

PMID: 25181483

Authors - Takeshi Yamasaki