SNX15 Monoclonal antibody, PBS Only

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  WB analysis using 68239-1-Ig (same clone as 68239-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68239-1-Ig (SNX15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68239-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68239-1-Ig (same clone as 68239-1-PBS)

  WB result of SNX15 antibody (68239-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX15 transfected HeLa cells. This data was developed using the same antibody clone with 68239-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68239-1-Ig (same clone as 68239-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68239-1-Ig (SNX15 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68239-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68239-1-Ig (same clone as 68239-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68239-1-Ig (SNX15 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68239-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 68239-1-Ig (same clone as 68239-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SNX15 antibody (68239-1-Ig, Clone: 1A10B7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68239-1-PBS in a different storage buffer formulation.

SNX15 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68239-1-PBS

Clone No.1A10B7

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Sorting nexin-15, sorting nexin 15, HSAF001435, 1A10B7

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Product Information

68239-1-PBS targets SNX15 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8839

  Product name: Recombinant human SNX15 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-342 aa of BC009897

  Sequence: MSRQAKDDFLRHYTVSDPRTHPKGYTEYKVTAQFISKKDPEDVKEVVVWKRYSDFRKLHGDLAYTHRNLFRRLEEFPAFPRAQVFGRFEASVIEERRKGAEDLLRFTVHIPALNNSPQLKEFFRGGEVTRPLEVSRDLHILPPPLIPTPPPDDPRLSQLLPAERRGLEELEVPVDPPPSSPAQEALDLLFNCESTEEASGSPARGPLTEAELALFDPFSKEEGAAPSPTHVAELATMEVESARLDQEPWEPGGQEEEEDGEGGPTPAYLSQATELITQALRDEKAGAYAAALQGYRDGVHVLLQGVPSDPLPARQEGVKKKAAEYLKRAEEILRLHLSQLPP

• Full Name: sorting nexin 15
• Calculated Molecular Weight: 342 aa, 38 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC009897
• Gene Symbol: SNX15
• Gene ID (NCBI): 29907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NRS6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SNX15 belongs to the sorting nexin family and is involved in several stages of intracellular trafficking. Overexpression of SNX15 results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits (PMID: 11085978). SNX15 is widely expressed, with the highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine.