Tested Applications
Positive WB detected in | HeLa cells, LNCaP cells |
Positive IF/ICC detected in | U2OS cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85909-2-RR targets SNX15 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | SNX15 fusion protein Ag9006 Predict reactive species |
Full Name | sorting nexin 15 |
Calculated Molecular Weight | 342 aa, 38 kDa |
Observed Molecular Weight | 40-50 kDa |
GenBank Accession Number | BC009897 |
Gene Symbol | SNX15 |
Gene ID (NCBI) | 29907 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9NRS6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SNX15 belongs to the sorting nexin family and is involved in several stages of intracellular trafficking. Overexpression of SNX15 results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits (PMID: 11085978). SNX15 is widely expressed, with the highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine.
Protocols
Product Specific Protocols | |
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WB protocol for SNX15 antibody 85909-2-RR | Download protocol |
IF protocol for SNX15 antibody 85909-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |