SNX15 Recombinant antibody

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  WB analysis using 85909-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85909-2-RR (SNX15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 85909-2-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SNX15 antibody (85909-2-RR, Clone: 250152D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SNX15 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85909-2-RR

Clone No.250152D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

HSAF001435, sorting nexin 15, Sorting nexin-15

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Tested Applications

• Positive WB detected in: HeLa cells, LNCaP cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85909-2-RR targets SNX15 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SNX15 fusion protein Ag9006
• Full Name: sorting nexin 15
• Calculated Molecular Weight: 342 aa, 38 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC009897
• Gene Symbol: SNX15
• Gene ID (NCBI): 29907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NRS6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNX15 belongs to the sorting nexin family and is involved in several stages of intracellular trafficking. Overexpression of SNX15 results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits (PMID: 11085978). SNX15 is widely expressed, with the highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine.