SNX18 Monoclonal antibody, PBS Only

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  WB analysis using 68305-1-Ig (same clone as 68305-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68305-1-Ig (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68305-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 68305-1-Ig (same clone as 68305-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68305-1-Ig (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68305-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68305-1-Ig (same clone as 68305-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SNX18 antibody (68305-1-Ig, Clone: 2E7D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68305-1-PBS in a different storage buffer formulation.

SNX18 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68305-1-PBS

Clone No.2E7D6

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FLJ11997, FLJ32560, FLJ61062, SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18

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Product Information

68305-1-PBS targets SNX18 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17388

  Product name: Recombinant human SNX18 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 55-284 aa of BC060791

  Sequence: YVQVIRAPEPGPAGDGGPGAPARYANVPPGGFEPLPVAPPASFKPPPDAFQALLQPQQAPPPSTFQPPGAGFPYGGGALQPSPQQLYGGYQASQGSDDDWDDEWDDSSTVADEPGALGSGAYPDLDGSSSAGVGAAGRYRLSTRSDLSLGSRGGSVPPQHHPSGPKSSATVSRNLNRFSTFVKSGGEAFVLGEASGFVKDGDKLCVVLGPYGPEWQENPYPFQCTIDDPT

• Full Name: sorting nexin 18
• Calculated Molecular Weight: 628 aa, 69 kDa
• Observed Molecular Weight: 67-70 kDa
• GenBank Accession Number: BC060791
• Gene Symbol: SNX18
• Gene ID (NCBI): 112574
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q96RF0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy, and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).