Tested Applications
| Positive WB detected in | COLO 320 cells, A549 cells, HeLa cells |
| Positive IHC detected in | human stomach tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10772-1-AP targets SNX3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1160 Product name: Recombinant human SNX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-162 aa of BC008444 Sequence: MAETVADTRRLITKPQNLNDAYGPPSNFLEIDVSNPQTVGVGRGRFTTYEIRVKTNLPIFKLKESTVRRRYSDFEWLRSELERESKVVVPPLPGKAFLRQLPFRGDDGIFDDNFIEERKQGLEQFINKVAGHPLAQNERCLHMFLQDEIIDKSYTPSKIRHA Predict reactive species |
| Full Name | sorting nexin 3 |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 16-20 kDa |
| GenBank Accession Number | BC008444 |
| Gene Symbol | SNX3 |
| Gene ID (NCBI) | 8724 |
| RRID | AB_2192691 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60493 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SNX3 is a member of the sorting nexin family of proteins regulating membrane traffic. SNX3 regulates endosomal function through its PX (phox homology)-domain-mediated interaction with PtdIns(3)P (PMID: 11433298). It is a potential regulator of neurite formation and may play an important role in the development and function of the central nervous system (PMID: 19576982; 20817026).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SNX3 antibody 10772-1-AP | Download protocol |
| IHC protocol for SNX3 antibody 10772-1-AP | Download protocol |
| WB protocol for SNX3 antibody 10772-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SNX3 Polyclonal antibody (catalog #10772-1-AP) has 13 citations. They appear in journals including Nature Cell Biology, Nature Communications, Cell Death & Differentiation, Cell Death & Disease, Oncogene, Acta Pharmaceutica Sinica B, Acta Pharmacologica Sinica, Environmental International, Cell Molecular Life Sciences, Biomedicines, Journal of Proteomics, Cell Structure & Function, and Journal of Cell Science. Research fields span endosomal/retromer trafficking, Wnt signaling, heart failure, ferroptosis, pulmonary fibrosis, breast cancer metastasis, autophagy, and viral infection.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Class III phosphatidylinositol-3-OH kinase controls epithelial integrity through endosomal LKB1 regulation.
| ||
Nat Commun SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion.
| ||
Acta Pharm Sin B Sorting nexin 3 exacerbates doxorubicin-induced cardiomyopathy via regulation of TFRC-dependent ferroptosis | ||
Cell Death Differ Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3.
| ||
Cell Death Dis Targeting sorting nexin 3 to treat pulmonary fibrosis by dual modulating Wnt/β-catenin signaling. | ||
Acta Pharmacol Sin SNX3 mediates heart failure by interacting with HMGB1 and subsequently facilitating its nuclear-cytoplasmic translocation |
Reviews
What customers say
The single review for 10772-1-AP (rated 4/5) was for Western Blot on MEFs at a 1:500 dilution. The reviewer, a long-time Proteintech customer, highlighted that the company's antibodies react with both mouse and human proteins, which they consider a significant advantage. Overall, the feedback is positive, emphasizing the product's cross-species reactivity and the reviewer's continued loyalty to the brand.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Rafeeq (Verified Customer) (01-30-2019) | I have been using Antibodies from Proteintech since two years. They have antibodies reactive to both mouse and human origin proteins. This is always a plus point.
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