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SNX3 Polyclonal antibody
SNX3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10772-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, A549 cells, HeLa cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
| CoIP | See 1 publications below |
Product Information
10772-1-AP targets SNX3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SNX3 fusion protein Ag1160 |
| Full Name | sorting nexin 3 |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 16-20 kDa |
| GenBank Accession Number | BC008444 |
| Gene Symbol | SNX3 |
| Gene ID (NCBI) | 8724 |
| RRID | AB_2192691 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SNX3 is a member of the sorting nexin family of proteins regulating membrane traffic. SNX3 regulates endosomal function through its PX (phox homology)-domain-mediated interaction with PtdIns(3)P (PMID: 11433298). It is a potential regulator of neurite formation and may play an important role in the development and function of the central nervous system (PMID: 19576982; 20817026).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SNX3 antibody 10772-1-AP | Download protocol |
| IHC protocol for SNX3 antibody 10772-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Class III phosphatidylinositol-3-OH kinase controls epithelial integrity through endosomal LKB1 regulation.
| ||
Acta Pharm Sin B Sorting nexin 3 exacerbates doxorubicin-induced cardiomyopathy via regulation of TFRC-dependent ferroptosis | ||
Nat Commun SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion.
| ||
Environ Int HIF-1α/m6A/NF-κB/CCL3 axis-mediated immunosurveillance participates in low level benzene-related erythrohematopoietic development toxicity | ||
Cell Death Differ Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3.
| ||
Oncogene EGF-SNX3-EGFR axis drives tumor progression and metastasis in triple-negative breast cancers.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Rafeeq (Verified Customer) (01-30-2019) | I have been using Antibodies from Proteintech since two years. They have antibodies reactive to both mouse and human origin proteins. This is always a plus point.
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