SNX3 Polyclonal antibody

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  WB analysis of COLO 320 using 10772-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10772-1-AP (SNX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10772-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10772-1-AP (SNX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10772-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10772-1-AP (SNX3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 10772-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10772-1-AP (SNX3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 10772-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10772-1-AP (SNX3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10772-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX3 antibody (10772-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  IF Staining of A431 using 10772-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SNX3 antibody (10772-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

SNX3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10772-1-AP

Publications(9)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

Grd19, MCOPS8, Protein SDP3, SDP3, sorting nexin 3

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Tested Applications

• Positive WB detected in: COLO 320 cells, A549 cells, HeLa cells
• Positive IHC detected in: human stomach tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 2 publications below

Product Information

10772-1-AP targets SNX3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1160

  Product name: Recombinant human SNX3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-162 aa of BC008444

  Sequence: MAETVADTRRLITKPQNLNDAYGPPSNFLEIDVSNPQTVGVGRGRFTTYEIRVKTNLPIFKLKESTVRRRYSDFEWLRSELERESKVVVPPLPGKAFLRQLPFRGDDGIFDDNFIEERKQGLEQFINKVAGHPLAQNERCLHMFLQDEIIDKSYTPSKIRHA

• Full Name: sorting nexin 3
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 16-20 kDa
• GenBank Accession Number: BC008444
• Gene Symbol: SNX3
• Gene ID (NCBI): 8724
• RRID: AB_2192691
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60493
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNX3 is a member of the sorting nexin family of proteins regulating membrane traffic. SNX3 regulates endosomal function through its PX (phox homology)-domain-mediated interaction with PtdIns(3)P (PMID: 11433298). It is a potential regulator of neurite formation and may play an important role in the development and function of the central nervous system (PMID: 19576982; 20817026).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Cell Biol; Class III phosphatidylinositol-3-OH kinase controls epithelial integrity through endosomal LKB1 regulation.; Authors - Fergal O'Farrell ; KD Validated
mouse	WB,IHC,IF,CoIP	Acta Pharm Sin B; Sorting nexin 3 exacerbates doxorubicin-induced cardiomyopathy via regulation of TFRC-dependent ferroptosis; Authors - Wenjing Yu
human	WB,IF	Nat Commun; SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion.; Authors - Ian J McGough ; KD Validated
mouse	WB	Environ Int; HIF-1α/m6A/NF-κB/CCL3 axis-mediated immunosurveillance participates in low level benzene-related erythrohematopoietic development toxicity; Authors - Xiaowei Cong
rat,mouse	WB,IF	Cell Death Differ; Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3.; Authors - Jing Lu ; KO Validated
human	WB,IF	Oncogene; EGF-SNX3-EGFR axis drives tumor progression and metastasis in triple-negative breast cancers.; Authors - Esra Cicek ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rafeeq (Verified Customer) (01-30-2019)

I have been using Antibodies from Proteintech since two years. They have antibodies reactive to both mouse and human origin proteins. This is always a plus point.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MEFs