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SNX5 Polyclonal antibody
SNX5 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, ELISA
Cat no : 17918-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293T cells, K-562 cells, mouse kidney tissue, human kidney tissue, Neuro-2a cells, U2OS cells, Jurkat cells|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 9 publications below|
|IHC||See 1 publications below|
|IP||See 1 publications below|
17918-1-AP targets SNX5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SNX5 fusion protein Ag12330|
|Full Name||sorting nexin 5|
|Calculated molecular weight||404 aa, 47 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC093623|
|Gene ID (NCBI)||27131|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX5 (Sorting nexin-5) was originally identified as a putative FANCA-binding protein (PMID: 10600472). SNX5 has a phox homology (PX) domain in the N-terminus. It is involved in several stages of intracellular trafficking. SNX5 is a component of the mammalian retromer complex, which is involved in recycling proteins from endosomes to the trans-Golgi network or plasma membrane (PMID: 17148574; 26199408).
|Product Specific Protocols|
|WB protocol for SNX5 antibody 17918-1-AP||Download protocol|
|IHC protocol for SNX5 antibody 17918-1-AP||Download protocol|
|IP protocol for SNX5 antibody 17918-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Nat Cell Biol
Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.
SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion.
Proc Natl Acad Sci U S A
Retromer dysfunction in amyotrophic lateral sclerosis.
J Cell Biol
Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.
Sorting nexin 1 loss results in increased oxidative stress and hypertension.
J Cell Sci
A heterodimeric SNX4--SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly.