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  • KD/KO Validated

SNX5 Polyclonal antibody

SNX5 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17918-1-AP

Synonyms

SNX5, sorting nexin 5



Tested Applications

Positive WB detected inHEK-293T cells, K-562 cells, mouse kidney tissue, human kidney tissue, Neuro-2a cells, U2OS cells, Jurkat cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17918-1-AP targets SNX5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX5 fusion protein Ag12330
Full Name sorting nexin 5
Calculated molecular weight 404 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC093623
Gene symbol SNX5
Gene ID (NCBI) 27131
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX5 (Sorting nexin-5) was originally identified as a putative FANCA-binding protein (PMID: 10600472). SNX5 has a phox homology (PX) domain in the N-terminus. It is involved in several stages of intracellular trafficking. SNX5 is a component of the mammalian retromer complex, which is involved in recycling proteins from endosomes to the trans-Golgi network or plasma membrane (PMID: 17148574; 26199408).

Protocols

Product Specific Protocols
WB protocol for SNX5 antibody 17918-1-APDownload protocol
IHC protocol for SNX5 antibody 17918-1-APDownload protocol
IP protocol for SNX5 antibody 17918-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.

Authors - Boris Simonetti
humanWB

Nat Commun

SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion.

Authors - Ian J McGough
mouseWB

Proc Natl Acad Sci U S A

Retromer dysfunction in amyotrophic lateral sclerosis.

Authors - Eduardo J Pérez-Torres
humanWB

J Cell Biol

Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.

Authors - Boris Simonetti
  • KO Validated
mouseWB

FASEB J

Sorting nexin 1 loss results in increased oxidative stress and hypertension.

Authors - Jian Yang
humanWB

J Cell Sci

A heterodimeric SNX4--SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly.

Authors - Zuriñe Antón