SNX5 Polyclonal antibody

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  WB analysis using 17918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 17918-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17918-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 17918-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 17918-1-AP

  IP result of anti-SNX5 (IP:17918-1-AP, 4ug; Detection:17918-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 17918-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17918-1-AP (SNX5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17918-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17918-1-AP (SNX5 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 17918-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17918-1-AP (SNX5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

SNX5 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17918-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Sorting nexin-5

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse kidney tissue, human kidney tissue, Neuro-2a cells, K-562 cells, Jurkat cells, U2OS cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

17918-1-AP targets SNX5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNX5 fusion protein Ag12330
• Full Name: sorting nexin 5
• Calculated Molecular Weight: 404 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC093623
• Gene Symbol: SNX5
• Gene ID (NCBI): 27131
• RRID: AB_2192708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5X3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX5 (Sorting nexin-5) was originally identified as a putative FANCA-binding protein (PMID: 10600472). SNX5 has a phox homology (PX) domain in the N-terminus. It is involved in several stages of intracellular trafficking. SNX5 is a component of the mammalian retromer complex, which is involved in recycling proteins from endosomes to the trans-Golgi network or plasma membrane (PMID: 17148574; 26199408).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.; Authors - Boris Simonetti
human	WB	Nat Commun; SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion.; Authors - Ian J McGough
mouse	WB	Proc Natl Acad Sci U S A; Retromer dysfunction in amyotrophic lateral sclerosis.; Authors - Eduardo J Pérez-Torres
human	WB	J Cell Biol; Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.; Authors - Boris Simonetti ; KO Validated
rat	WB	Oxid Med Cell Longev; Sorting Nexin 5 Plays an Important Role in Promoting Ferroptosis in Parkinson's Disease.; Authors - Zifeng Huang ; KD Validated
human	WB	Cells; Five Inhibitory Receptors Display Distinct Vesicular Distributions in Murine T Cells; Authors - Jiahe Lu