SNX7 Polyclonal antibody

• 

  WB analysis of HeLa using 12269-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12269-1-AP (SNX7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 12269-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12269-1-AP (SNX7 antibody) at dilution of 1:50 (under 10x lens).

• Featured Product
• KD/KO Validated

SNX7 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12269-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

SNX7, sorting nexin 7

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IP: See 1 publications below

Product Information

12269-1-AP targets SNX7 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2920

  Product name: Recombinant human SNX7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-337 aa of BC018105

  Sequence: MDMNSFSPMMPTSPLSMINQIKFEDEPDLKDLFITVDEPESHVTTIETFITYRIITKTSRGEFDSSEFEVRRRYQDFLWLKGKLEEAHPTLIIPPLPEKFIVKGMVERFNDDFIETRRKALHKFLNRIADHPTLTFNEDFKIFLTAQAWELSSHKKQGPGLLSRMGQTVRAVASSMRGVKNRPEEFMEMNNFIELFSQKINLIDKISQRIYKEEREYFDEMKEYGPIHILWSASEEDLVDTLKDVASCIDRCCKATEKRMSGLSEALLPVVHEYVLYSEMLMGVMKRRDQIQAELDSKVEVLTYKKADTDLCLATWESFLTSQTNLHLEEASEDKP

• Full Name: sorting nexin 7
• Calculated Molecular Weight: 45 kDa, 39 kDa, 52 kDa
• Observed Molecular Weight: 45 kDa, 52 kDa
• GenBank Accession Number: BC018105
• Gene Symbol: SNX7
• Gene ID (NCBI): 51375
• RRID: AB_10694667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UNH6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	J Cell Sci; A heterodimeric SNX4:SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly; Authors - Zuriñe Antón ; KD Validated
human	WB	J Cell Sci; A heterodimeric SNX4--SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly.; Authors - Zuriñe Antón
	WB	Brain Res Bull; Deciphering Glioblastoma Pathogenesis: Insights from Mitophagy Dysregulation and SNX7 as a Therapeutic Target; Authors - Yuanlong Zhang