SOAT1/ACAT1 Polyclonal antibody

SOAT1/ACAT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13202-1-AP

Synonyms

ACACT, ACACT1, ACAT, ACAT 1, ACAT1, Cholesterol acyltransferase 1, RP11 215I23.2, SOAT, SOAT1, STAT, sterol O acyltransferase 1

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Validation Data Gallery

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  WB analysis using 13202-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13202-1-AP (SOAT1/ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HuH-7 cells, SW480 cells, THP-1 cells, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13202-1-AP targets SOAT1/ACAT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOAT1/ACAT1 fusion protein Ag3944
• Full Name: sterol O-acyltransferase 1
• Calculated Molecular Weight: 550 aa, 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC028940
• Gene Symbol: SOAT1
• Gene ID (NCBI): 6646
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sterol O-acyltransferase 1 (SOAT1/ACAT1) catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol (PubMed:16154994,16647063, 32433614, 32944968). It plays a role in lipoprotein assembly and dietary cholesterol absorption (PubMed:16154994, 9020103). SOAT1 has three isoforms with molecular weights of 65, 58, and 57 kDa.