SOCS3 Polyclonal antibody

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  WB analysis of HeLa using 14025-1-AP

  WB result of SOCS3 antibody (14025-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOCS3 transfected HeLa cells.

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  WB analysis using 14025-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14025-1-AP (SOCS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14025-1-AP

  IP result of anti-SOCS3 (IP:14025-1-AP, 4ug; Detection:14025-1-AP 1:500) with K-562 cells lysate 1720ug.

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  IHC staining of human skin cancer using 14025-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14025-1-AP (SOCS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

SOCS3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14025-1-AP

Publications(70)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

SSI3, SOCS-3, Cytokine-inducible SH2 protein 3, CIS-3, CIS3

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 61 publications below
• IHC: See 12 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

14025-1-AP targets SOCS3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOCS3 fusion protein Ag5170
• Full Name: suppressor of cytokine signaling 3
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC060858
• Gene Symbol: SOCS3
• Gene ID (NCBI): 9021
• RRID: AB_10597854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14543
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The suppressor of cytokine signaling (SOCS) family proteins are negative feedback system that inhibits cytokine signal transduction. One of the SOCS family, SOCS3, also named CIS3 and SSI3, is involved in inhibition of JAK/STAT pathway which regulates IL-6 signaling in vivo. SOCS3 can be rapidly trigger by a number of factors including interleukins, IFN-gamma and TNF-alpha. Pathology such as inflammatory response, wound repair and obesity and so on have been linked to SOCS3. Observed MW of SOCS3 is from 22-30 kDa(PMID: 24827536,PMID: 26260587, PMID: 26797799), consistent with the result of Catalog#14025-1-AP.

Publications

Species	Application	Title
human	WB	Redox Biol; TNFα counteracts interleukin-10 anti-inflammatory pathway through the NOX2-Lyn-SHP-1 axis in human monocytes; Authors - Marwa Ben-Khemis
mouse	WB	Cell Death Differ; TLR2-dependent selective autophagy regulates NF-κB lysosomal degradation in hepatoma-derived M2 macrophage differentiation.; Authors - Chang C-P CP
mouse	WB,IHC	Kidney Int; Ablation of proximal tubular suppressor of cytokine signaling 3 enhances tubular cell cycling and modifies macrophage phenotype during acute kidney injury.; Authors - Nathan Susnik
mouse	WB	Oncoimmunology; Transcriptional factor EB regulates macrophage polarization in the tumor microenvironment.; Authors - Liang Fang
human	WB	PLoS Pathog; Negative regulation of type I interferon signaling by integrin-linked kinase permits dengue virus replication; Authors - Yi-Sheng Kao
mouse	WB	PLoS Genet; PMID: 27711113; Authors - Xiaolin Lin