SOD1 Monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA sample test of MP50052-1

  Plasma of sixteen individual healthy human donors was measured. The SOD1 concentration of detected samples was determined to be 10,853.0 pg/mL with a range of 1,193.1-34,539.7 pg/mL.

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  Sandwich ELISA standard curve of MP50052-1

  Sandwich ELISA standard curve of MP50052-1, SOD1 Monoclonal Matched Antibody Pair - PBS only. 67480-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag28553. 67480-3-PBS was biotinylated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sandwich ELISA standard curve of MP50052-2

  Sandwich ELISA standard curve of MP50052-2, SOD1 Monoclonal Matched Antibody Pair - PBS only. 67480-4-PBS was coated to a plate as the capture antibody and inc4bated with serial dilutions of standard  Ag28553. 67480-3-PBS was biotinylated as the detection antibody. Range: 0.156-10 ng/mL.

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  Cytometric bead array sample test of MP50052-1

  Sample test of MP50052-1, SOD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67480-2-PBS. Detection antibody: 67480-3-PBS. Cell lysate(30μg/well): HEK293, Jurkat, K-562, HSC-T6. Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

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  Cytometric bead array standard curve of MP50052-1

  Cytometric bead array standard curve of MP50052-1, SOD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67480-2-PBS. Detection antibody: 67480-3-PBS. Standard:Ag28553. Range: 0.098-100 ng/mL.

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  Cytometric bead array standard curve of MP50052-2

  Cytometric bead array standard curve of MP50052-2, SOD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67480-4-PBS. Detection antibody: 67480-3-PBS. Standard:Ag28553. Range: 0.391-100 ng/mL.

SOD1 Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 67480-3-PBS

Clone No.5B1G3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

ALS1, EC:1.15.1.1, homodimer, hSod1, IPOA

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Product Information

67480-3-PBS targets SOD1 as part of a matched antibody pair:

MP50052-1: 67480-2-PBS capture and 67480-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP50052-2: 67480-4-PBS capture and 67480-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=1.81 x 10^-10M
  K_Off=3.56 x 10^-6M
  K_On=1.97 x 10^4M
• Immunogen:

  CatNo: Ag28553

  Product name: Recombinant human SOD1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-154 aa of BC001034

  Sequence: MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ

• Full Name: superoxide dismutase 1, soluble
• Calculated Molecular Weight: 16 kDa
• GenBank Accession Number: BC001034
• Gene Symbol: SOD1
• Gene ID (NCBI): 6647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00441
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.