SOD3 Polyclonal antibody

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SOD3 Polyclonal Antibody for WB, IP, ELISA

Cat No. 14316-1-AP Copied!

Publications (13)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, IP, ELISA

Synonyms

EC SOD, Extracellular superoxide dismutase, SOD3, Superoxide Dismutase 3

• 

  WB analysis using 14316-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14316-1-AP (SOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human heart using 14316-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14316-1-AP (SOD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of human placenta using 14316-1-AP

  IP result of anti-SOD3 (IP:14316-1-AP, 4ug; Detection:14316-1-AP 1:1000) with human placenta tissue lysate 2800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human urine sample, human heart tissue, human urine, human placenta tissue
• Positive IP detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14316-1-AP targets SOD3 in WB, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5556

  Product name: Recombinant human SOD3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-240 aa of BC014418

  Sequence: MLALLCSCLLLAAGASDAWTGEDSAEPNSDSAEWIRDMYAKVTEIWQEVMQRRDDDGTLHAACQVQPSATLDAAQPRVTGVVLFRQLAPRAKLDAFFALEGFPTEPNSSSRAIHVHQFGDLSQGCESTGPHYNPLAVPHPQHPGDFGNFAVRDGSLWRYRAGLAASLAGPHSIVGRAVVVHAGEDDLGRGGNQASVENGNAGRRLACCVVGVCGPGLWERQAREHSERKKRRRESECKAA

• Full Name: superoxide dismutase 3, extracellular
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 25-33 kDa
• GenBank Accession Number: BC014418
• Gene Symbol: SOD3
• Gene ID (NCBI): 6649
• RRID: AB_10898170
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08294
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOD is located intracellular (SOD-1; cytosolic, SOD-2; mitochondrial matrix) and extracellular (SOD-3), and catalyzes the dismutation of superoxide anion to H2O2. SOD3, or EC-SOD, is the most recently characterized SOD, exists as a copper and zinc-containing tetramer, and is synthesized containing a signal peptide that directs this enzyme exclusively to extracellular spaces. SOD-3 is a Mn-containing tetrameric protein with a subunit Mr of 24-35 kDa. The activity of EC-SOD is dependent on its ability to form a tetramer of 135 kDa.

Publications

What published studies show

SOD3 Polyclonal antibody (Cat# 14316-1-AP) has 13 citations across journals including Science Advances, British Journal of Pharmacology, Scientific Reports, Clinical Immunology, Journal of Proteome Research, Toxicology and Applied Pharmacology, Experimental Cell Research, Gene, International Journal of Stem Cells, Journal of Molecular Neuroscience, Translational Pediatrics, American Journal of Translational Research, and eLife. Research fields span oxidative stress, cancer (colorectal, lung, glioblastoma), pulmonary fibrosis, COPD, neurology, stem cell biology, metabolism, and proteomics.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Sci Adv; Synergistic antioxidant and gene supplementation for high-efficacy retinitis pigmentosa therapy.; Authors - Fuhua Wu
mouse,human	WB	Br J Pharmacol; Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis.; Authors - Ai Wei
	WB	Sci Rep; The double inhibition of PDK1 and STAT3-Y705 prevents liver metastasis in colorectal cancer.; Authors - Wenjuan Qin
human	WB	Clin Immunol; Oxidative stress is involved in immunosuppression and macrophage regulation in glioblastoma; Authors - Xisong Liang ; KD Validated
mouse	WB	J Proteome Res; Sex-Specific Alterations in Placental Proteomics Induced by Intrauterine Hyperglycemia; Authors - Zhuo-Ran Ren
human,rat	WB	Toxicol Appl Pharmacol; (-)-Epicatechin ameliorates cigarette smoke-induced lung inflammation via inhibiting ROS/NLRP3 inflammasome pathway in rats with COPD.; Authors - Xue Tian