SORBS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SORBS2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 24643-1-AP

Synonyms

Arg/Abl interacting protein 2, ARGBP2, KIAA0777, nArgBP2, PRO0618, Sorbin, SORBS2

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Validation Data Gallery

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  WB analysis using 24643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 24643-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDCK using 24643-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 24643-1-AP

  IP result of anti-SORBS2 (IP:24643-1-AP, 4ug; Detection:24643-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 24643-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24643-1-AP (SORBS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 24643-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 24643-1-AP (SORBS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A2780 cells, MDCK cells, U-251 cells, mouse pancreas tissue, rat pancreas tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse heart tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 3 publications below

Product Information

24643-1-AP targets SORBS2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, canine samples.

• Tested Reactivity: human, mouse, canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SORBS2 fusion protein Ag20041
• Full Name: sorbin and SH3 domain containing 2
• Calculated Molecular Weight: 1100 aa, 124 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC011883
• Gene Symbol: SORBS2
• Gene ID (NCBI): 8470
• RRID: AB_2879652
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SORBS2, also known as ARGBP2, has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. SORBS2 is widely expressed in human tissues and extremely abundant in the heart. In epithelial cells SORBS2 is located in stress fibers and the nucleus; In cardiac muscle cells. SORBS2 is located in the Z-disks of sarcomeres. The subcellular localization suggests that SORBS2 functions as an adapter protein to assemble signaling complexes in stress fibers, and may influence the contractile or elastic properties of cardiac sarcomeres(PMID: 9211900). Alternate splicing results in multiple transcript variants( PMID: 28961272).

Publications

Species	Application	Title
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
human	WB,IHC,IF,IP	EBioMedicine; Elevated myocardial SORBS2 and the underlying implications in left ventricular noncompaction cardiomyopathy.; Authors - Chunyan Li
human,mouse	WB,IP	Aging (Albany NY); The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.; Authors - Chunyan Li
human	WB, IP	J Biol Chem; Arg Kinase Binding Protein 2 (ArgBP2) interaction with α-actinin and actin stress fibres inhibits cell migration.; Authors - Praju Vikas Anekal
human	WB	J Transl Med; SORBS2 as a molecular target for atherosclerosis in patients with familial hypercholesterolemia.; Authors - Ming-Ming Liu
human	WB	Mol Med Rep; Musk ketone induces apoptosis of gastric cancer cells via downregulation of sorbin and SH3 domain containing 2.; Authors - Juan An ; KD Validated