SOS1 Recombinant monoclonal antibody

• 

  WB analysis using 82784-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82784-2-RR (SOS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

SOS1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 82784-2-RR

Clone No.2J16

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

2J16, Son of sevenless homolog 1, SOS 1, SOS-1

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, K-562 cells, NIH/3T3 cells, THP-1 cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82784-2-RR targets SOS1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: son of sevenless homolog 1 (Drosophila)
• Calculated Molecular Weight: 152 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: NM_005633
• Gene Symbol: SOS1
• Gene ID (NCBI): 6654
• RRID: AB_3670550
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q07889
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOS1 promotes the exchange of Ras-bound GDP by GTP. EPS8, E3B1 and SOS1 form a tricomplex in vivo that exhibits RAC-specific guanine nucleotide exchange factor activity in vitro. Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1). Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4). The antibody is specific to SOS1.