SOX12 Polyclonal antibody

• Featured Product
• KD/KO Validated

SOX12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 23939-1-AP

Synonyms

Protein SOX 22, SOX12, SOX22, Transcription factor SOX 12

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Validation Data Gallery

• 

  WB analysis of mouse testis using 23939-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23939-1-AP (SOX12 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of BxPC-3 using 23939-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 23939-1-AP (SOX12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23939-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23939-1-AP (SOX12 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse testis using 23939-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23939-1-AP (SOX12 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse brain tissue, BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

The immunogen of 23939-1-AP is SOX12 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOX12 fusion protein Ag21043
• Full Name: SRY (sex determining region Y)-box 12
• Calculated Molecular Weight: 315 aa, 34 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC067361
• Gene Symbol: SOX12
• Gene ID (NCBI): 6666
• RRID: AB_2879368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Mol Ther Nucleic Acids; lncRNA PRR34-AS1 promotes HCC development via modulating Wnt/β-catenin pathway by absorbing miR-296-5p and upregulating E2F2 and SOX12.; Authors - Minzhen Qin
human	WB	Cancer Gene Ther; LncRNA UCA1 promotes SOX12 expression in breast cancer by regulating m6A modification of miR-375 by METTL14 through DNA methylation.; Authors - Chengpeng Zhao
human	WB	Exp Cell Res; LncRNA LINC00680 promotes lung adenocarcinoma growth via binding to GATA6 and canceling GATA6-mediated suppression of SOX12 expression.; Authors - Xiaojuan Sun ; KD Validated
human	WB	Exp Cell Res; SOX12 promotes the growth of multiple myeloma cells by enhancing Wnt/β-catenin signaling.; Authors - Ying Gao ; KD Validated
human	WB,RIP	Environ Toxicol; Long noncoding RNA DUXAP10 promotes the stemness of glioma cells by recruiting HuR to enhance Sox12 mRNA stability.; Authors - Bolin Wu ; KD Validated
human	WB	Ann Transl Med; The lncRNA TERC promotes gastric cancer cell proliferation, migration, and invasion by sponging miR-423-5p to regulate SOX12 expression; Authors - Jianhua Wang