SOX13 Polyclonal antibody

• Featured Product
• KD/KO Validated

SOX13 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 18902-1-AP

Synonyms

ICA12, Islet cell antigen 12, Sox 13, SOX13, Transcription factor SOX 13

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Validation Data Gallery

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  WB analysis using 18902-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18902-1-AP (SOX13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 18902-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 18902-1-AP (SOX13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of BGC-823 using 18902-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 18902-1-AP (SOX13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 18902-1-AP

  IP Result of anti-SOX13 (IP:18902-1-AP, 4ug; Detection:18902-1-AP 1:500) with SH-SY5Y cells lysate 1200ug.

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  IF Staining of HeLa using 18902-1-AP

  Immunofluorescent analysis of Hela cells, using SOX13 antibody 18902-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: BGC-823 cells, NCI-H1299 cells
• Positive IP detected in: SH-SY5Y cells
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Product Information

18902-1-AP targets SOX13 in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOX13 fusion protein Ag13537
• Full Name: SRY (sex determining region Y)-box 13
• Calculated molecular weight: 622 aa, 69 kDa
• Observed molecular weight: 69 kDa
• GenBank accession number: BC106038
• Gene symbol: SOX13
• Gene ID (NCBI): 9580
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRY-BOX 13(SOX13), identified a sequence homologous more than 60% to the SRY HMG box ,with a centrally located HMG box and an N-terminal leucine zipper motif with a neighboring glutamine stretch called a Q box. SOX13 binds to the consensus HMG-box motif. It's an IDDM-specific human autoantigen, and a gamma-delta-specific gene in the immune system. It can modulates Wnt/TCF activity. This is a rabbit polyclonal raised against part of N-terminal chain of SOX13 of human origin.

Publications

Species	Application	Title
human	WB,IF	Front Cardiovasc Med; Sox13 is a novel flow-sensitive transcription factor that prevents inflammation by repressing chemokine expression in endothelial cells; Authors - Catherine Demos ; KD Validated
human	WB,IHC	Lab Invest; Sox13 promotes hepatocellular carcinoma metastasis by transcriptionally activating Twist1.; Authors - Min Feng ; KD Validated
mouse	WB, IF	Mol Cell Biochem; Expressions of Sox9, Sox5, and Sox13 transcription factors in mice testis during postnatal development.; Authors - Mikella Daigle
human	WB,IHC	Bioengineered; Sex-determining Region Y-box transcription factor 13 promotes breast cancer cell proliferation and glycolysis by activating the tripartite motif containing 11-mediated Wnt/β-catenin signaling pathway.; Authors - Xiaoyan Jin ; KD Validated
human	WB,chIP	J Exp Clin Cancer Res; FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma.; Authors - Zhenwei He